%0 Journal Article %1 RN1191 %A Schönknecht, G. %A Spoormaker, P. %A Steinmeyer, R. %A Brüggeman, L. %A Ache, P. %A Dutta, R. %A Reintanz, B. %A Godde, M. %A Hedrich, R. %A Palme, K. %D 2002 %J Febs Letters %K channel domain k+ myOwn two-pore %N 1-3 %P 28-32 %R Doi 10.1016/S0014-5793(01)03273-2 %T KCO1 is a component of the slow-vacuolar (SV) ion channel %U /brokenurl#<Go to ISI>://WOS:000173628000006 %V 511 %X The Arabidopsis double pore K+ channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT-PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles; from Arabidopsis wild-type and kco1 knockout plants were isolated for patch-clamp analyses. Currents mediated by slow-activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild-type. This shown that KCO1 is involved in the formation of SV channels. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

@article{RN1191, abstract = {The Arabidopsis double pore K+ channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT-PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles; from Arabidopsis wild-type and kco1 knockout plants were isolated for patch-clamp analyses. Currents mediated by slow-activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild-type. This shown that KCO1 is involved in the formation of SV channels. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Schönknecht, G. and Spoormaker, P. and Steinmeyer, R. and Brüggeman, L. and Ache, P. and Dutta, R. and Reintanz, B. and Godde, M. and Hedrich, R. and Palme, K.}, biburl = {https://www.bibsonomy.org/bibtex/2396192dbb4cedf5ac9642377a81a16e7/rainerhedrich_2}, doi = {Doi 10.1016/S0014-5793(01)03273-2}, interhash = {c16fb54b94f59afe87f3e0c2e3914e5d}, intrahash = {396192dbb4cedf5ac9642377a81a16e7}, issn = {0014-5793}, journal = {Febs Letters}, keywords = {channel domain k+ myOwn two-pore}, note = {517uf Times Cited:49 Cited References Count:27}, number = {1-3}, pages = {28-32}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {KCO1 is a component of the slow-vacuolar (SV) ion channel}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000173628000006}, volume = 511, year = 2002 }