%0 Journal Article %1 david2019regenerative %A David, Penuel P. %A Santiago, Cecilia D. %A Castillo, Anges L. %A De Guzman, Gerard Q. %D 2019 %E JPPRes, %J Journal of Pharmacy & Pharmacognosy Research %K Equisetum-hyemale alopecia flavonoids hair-regenerative horsetail %N 5 %P 323-330 %T Hair regenerative activities of flavonoid-rich extract of Equisetum hyemale L. (Equisetaceae) in chemically-induced alopecia in Sprague Dawley rats %U http://jppres.com/jppres/pdf/vol7/jppres19.557_7.5.323.pdf %V 7 %X Context: The prevalence of alopecia around the world is high and awareness continuously increased due to social stigma. There are limited commercially-available medications for the management of different forms of baldness, most of which are prohibitively expensive and presents with various adverse effects. Aims: To evaluate the flavonoid-rich ethyl acetate extract of Equisetum hyemale stem for its hair-regenerative properties in chemically-induced alopecia in Sprague Dawley rats. Methods: Various concentrations of the flavonoid-rich extract of E. hyemale were applied in depilated areas in dorsal rat skin after chemical induction of alopecia. Evidence of hair growth was observed for 28 days in a weekly basis. Histopathological analysis of rat skin was performed to demonstrate evidence of follicular hair growth. Results: The abundance of flavonoids in the ethyl acetate extract was established by total flavonoid contents and LC-MS analyses. The 2.5% E. hyemale extract exhibited hair-regenerative properties with high tensile strengths, combined masses and follicular growth, which was comparable to the positive control, 5% minoxidil (p>0.05). The highest rate of hair follicular development was observed in the telogen phase in rat skin after 28 days of treatment. Conclusions: The flavonoid-rich ethyl acetate extract of E. hyemale exhibits in situ hair-regenerative properties in chemically-induced alopecia in rats at 2.5% w/v concentration.

@article{david2019regenerative, abstract = {Context: The prevalence of alopecia around the world is high and awareness continuously increased due to social stigma. There are limited commercially-available medications for the management of different forms of baldness, most of which are prohibitively expensive and presents with various adverse effects. Aims: To evaluate the flavonoid-rich ethyl acetate extract of Equisetum hyemale stem for its hair-regenerative properties in chemically-induced alopecia in Sprague Dawley rats. Methods: Various concentrations of the flavonoid-rich extract of E. hyemale were applied in depilated areas in dorsal rat skin after chemical induction of alopecia. Evidence of hair growth was observed for 28 days in a weekly basis. Histopathological analysis of rat skin was performed to demonstrate evidence of follicular hair growth. Results: The abundance of flavonoids in the ethyl acetate extract was established by total flavonoid contents and LC-MS analyses. The 2.5% E. hyemale extract exhibited hair-regenerative properties with high tensile strengths, combined masses and follicular growth, which was comparable to the positive control, 5% minoxidil (p>0.05). The highest rate of hair follicular development was observed in the telogen phase in rat skin after 28 days of treatment. Conclusions: The flavonoid-rich ethyl acetate extract of E. hyemale exhibits in situ hair-regenerative properties in chemically-induced alopecia in rats at 2.5% w/v concentration.}, added-at = {2020-01-19T04:38:38.000+0100}, author = {David, Penuel P. and Santiago, Cecilia D. and Castillo, Anges L. and De Guzman, Gerard Q.}, biburl = {https://www.bibsonomy.org/bibtex/2ee3c9e8c0a3e2134d84eabeceeb76ba1/jppres}, editor = {JPPRes}, interhash = {c1d8f3c692dba7af844c832d9262ab5b}, intrahash = {ee3c9e8c0a3e2134d84eabeceeb76ba1}, issn = {0719-4250}, journal = {Journal of Pharmacy & Pharmacognosy Research}, keywords = {Equisetum-hyemale alopecia flavonoids hair-regenerative horsetail}, language = {Eng}, month = {Sep-Oct}, number = 5, pages = {323-330}, timestamp = {2020-01-19T04:38:38.000+0100}, title = {Hair regenerative activities of flavonoid-rich extract of Equisetum hyemale L. (Equisetaceae) in chemically-induced alopecia in Sprague Dawley rats}, url = {http://jppres.com/jppres/pdf/vol7/jppres19.557_7.5.323.pdf}, volume = 7, year = 2019 }