%0 Journal Article %1 Annaluru2014Total %A Annaluru, Narayana %A Muller, Héloïse %A Mitchell, Leslie A. %A Ramalingam, Sivaprakash %A Stracquadanio, Giovanni %A Richardson, Sarah M. %A Dymond, Jessica S. %A Kuang, Zheng %A Scheifele, Lisa Z. %A Cooper, Eric M. %A Cai, Yizhi %A Zeller, Karen %A Agmon, Neta %A Han, Jeffrey S. %A Hadjithomas, Michalis %A Tullman, Jennifer %A Caravelli, Katrina %A Cirelli, Kimberly %A Guo, Zheyuan %A London, Viktoriya %A Yeluru, Apurva %A Murugan, Sindurathy %A Kandavelou, Karthikeyan %A Agier, Nicolas %A Fischer, Gilles %A Yang, Kun %A Martin, J. Andrew %A Bilgel, Murat %A Bohutskyi, Pavlo %A Boulier, Kristin M. %A Capaldo, Brian J. %A Chang, Joy %A Charoen, Kristie %A Choi, Woo J. %A Deng, Peter %A DiCarlo, James E. %A Doong, Judy %A Dunn, Jessilyn %A Feinberg, Jason I. %A Fernandez, Christopher %A Floria, Charlotte E. %A Gladowski, David %A Hadidi, Pasha %A Ishizuka, Isabel %A Jabbari, Javaneh %A Lau, Calvin Y. L. %A Lee, Pablo A. %A Li, Sean %A Lin, Denise %A Linder, Matthias E. %A Ling, Jonathan %A Liu, Jaime %A Liu, Jonathan %A London, Mariya %A Ma, Henry %A Mao, Jessica %A McDade, Jessica E. %A McMillan, Alexandra %A Moore, Aaron M. %A Oh, Won C. %A Ouyang, Yu %A Patel, Ruchi %A Paul, Marina %A Paulsen, Laura C. %A Qiu, Judy %A Rhee, Alex %A Rubashkin, Matthew G. %A Soh, Ina Y. %A Sotuyo, Nathaniel E. %A Srinivas, Venkatesh %A Suarez, Allison %A Wong, Andy %A Wong, Remus %A Xie, Wei R. %A Xu, Yijie %A Yu, Allen T. %A Koszul, Romain %A Bader, Joel S. %A Boeke, Jef D. %A Chandrasegaran, Srinivasan %D 2014 %I American Association for the Advancement of Science %J Science %K minimal-genome synthetic-biology %N 6179 %P 55--58 %R 10.1126/science.1249252 %T Total Synthesis of a Functional Designer Eukaryotic Chromosome %U http://dx.doi.org/10.1126/science.1249252 %V 344 %X One of the ultimate aims of synthetic biology is to build designer organisms from the ground up. Rapid advances in DNA synthesis has allowed the assembly of complete bacterial genomes. Eukaryotic organisms, with their generally much larger and more complex genomes, present an additional challenge to synthetic biologists. Annaluru et al. (p. 55, published online 27 March) designed a synthetic eukaryotic chromosome based on yeast chromosome III. The designer chromosome, shorn of destabilizing transfer RNA genes and transposons, is ∼14\% smaller than its wild-type template and is fully functional with every gene tagged for easy removal.

@article{Annaluru2014Total, abstract = {One of the ultimate aims of synthetic biology is to build designer organisms from the ground up. Rapid advances in {DNA} synthesis has allowed the assembly of complete bacterial genomes. Eukaryotic organisms, with their generally much larger and more complex genomes, present an additional challenge to synthetic biologists. Annaluru et al. (p. 55, published online 27 March) designed a synthetic eukaryotic chromosome based on yeast chromosome {III}. The designer chromosome, shorn of destabilizing transfer {RNA} genes and transposons, is ∼14\% smaller than its wild-type template and is fully functional with every gene tagged for easy removal.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Annaluru, Narayana and Muller, H\'{e}lo\"{i}se and Mitchell, Leslie A. and Ramalingam, Sivaprakash and Stracquadanio, Giovanni and Richardson, Sarah M. and Dymond, Jessica S. and Kuang, Zheng and Scheifele, Lisa Z. and Cooper, Eric M. and Cai, Yizhi and Zeller, Karen and Agmon, Neta and Han, Jeffrey S. and Hadjithomas, Michalis and Tullman, Jennifer and Caravelli, Katrina and Cirelli, Kimberly and Guo, Zheyuan and London, Viktoriya and Yeluru, Apurva and Murugan, Sindurathy and Kandavelou, Karthikeyan and Agier, Nicolas and Fischer, Gilles and Yang, Kun and Martin, J. Andrew and Bilgel, Murat and Bohutskyi, Pavlo and Boulier, Kristin M. and Capaldo, Brian J. and Chang, Joy and Charoen, Kristie and Choi, Woo J. and Deng, Peter and DiCarlo, James E. and Doong, Judy and Dunn, Jessilyn and Feinberg, Jason I. and Fernandez, Christopher and Floria, Charlotte E. and Gladowski, David and Hadidi, Pasha and Ishizuka, Isabel and Jabbari, Javaneh and Lau, Calvin Y. L. and Lee, Pablo A. and Li, Sean and Lin, Denise and Linder, Matthias E. and Ling, Jonathan and Liu, Jaime and Liu, Jonathan and London, Mariya and Ma, Henry and Mao, Jessica and McDade, Jessica E. and McMillan, Alexandra and Moore, Aaron M. and Oh, Won C. and Ouyang, Yu and Patel, Ruchi and Paul, Marina and Paulsen, Laura C. and Qiu, Judy and Rhee, Alex and Rubashkin, Matthew G. and Soh, Ina Y. and Sotuyo, Nathaniel E. and Srinivas, Venkatesh and Suarez, Allison and Wong, Andy and Wong, Remus and Xie, Wei R. and Xu, Yijie and Yu, Allen T. and Koszul, Romain and Bader, Joel S. and Boeke, Jef D. and Chandrasegaran, Srinivasan}, biburl = {https://www.bibsonomy.org/bibtex/27db23d1c8b22b20e8f79376d1ffc3f16/karthikraman}, citeulike-article-id = {13119064}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1249252}, citeulike-linkout-1 = {http://www.sciencemag.org/content/early/2014/03/26/science.1249252.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/early/2014/03/26/science.1249252.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/344/6179/55}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/24674868}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=24674868}, day = 04, doi = {10.1126/science.1249252}, interhash = {c36473bbc9517923af5c39d07953712c}, intrahash = {7db23d1c8b22b20e8f79376d1ffc3f16}, issn = {1095-9203}, journal = {Science}, keywords = {minimal-genome synthetic-biology}, month = apr, number = 6179, pages = {55--58}, pmid = {24674868}, posted-at = {2014-04-01 12:25:38}, priority = {2}, publisher = {American Association for the Advancement of Science}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Total Synthesis of a Functional Designer Eukaryotic Chromosome}, url = {http://dx.doi.org/10.1126/science.1249252}, volume = 344, year = 2014 }