%0 Journal Article %1 taha_multicenter_2010 %A Taha, Muhamed-Kheir %A Hedberg, Sara Thulin %A Szatanik, Marek %A Hong, Eva %A Ruckly, Corinne %A Abad, Raquel %A Bertrand, Sophie %A Carion, Francoise %A Claus, Heike %A Corso, Alejandra %A Enriquez, Rocio %A Heuberger, Sigrid %A Hryniewicz, Waleria %A Jolley, Keith A. %A Kriz, Paula %A Mollerach, Marta %A Musilek, Martin %A Neri, Arianna %A Olcen, Per %A Pana, Marina %A Skoczynska, Anna %A Pereira, Cecilia Sorhouet %A Stefanelli, Paola %A Tzanakaki, Georgina %A Unemo, Magnus %A Vazquez, Julio A. %A Vogel, Ulrich %A Wasko, Izabela %D 2010 %J Antimicrob. Agents Chemother. %K imported %N 9 %P 3651--3658 %R 10.1128/AAC.00315-10 %T Multicenter Study for Defining the Breakpoint for Rifampin Resistance in Neisseria meningitidis by rpoB Sequencing %U http://aac.asm.org/cgi/content/abstract/54/9/3651 %V 54 %X Identification of clinical isolates of Neisseria meningitidis that are resistant to rifampin is important to avoid prophylaxis failure in contacts of patients, but it is hindered by the absence of a breakpoint for resistance, despite many efforts toward standardization. We examined a large number (n = 392) of clinical meningococcal isolates, spanning 25 years (1984 to 2009), that were collected in 11 European countries, Argentina, and the Central African Republic. The collection comprises all clinical isolates with MICs of ≥0.25 mg/liter (n = 161) received by the national reference laboratories for meningococci in the participating countries. Representative isolates displaying rifampin MICs of \textless0.25 mg/liter were also examined (n = 231). Typing of isolates was performed, and a 660-bp DNA fragment of the rpoB gene was sequenced. Sequences differing by at least one nucleotide were defined as unique rpoB alleles. The geometric mean of the MICs was calculated for isolates displaying the same allele. The clinical isolates displaying rifampin MICs of \textgreater1 mg/liter possessed rpoB alleles with nonsynonymous mutations at four critical amino acid residues, D542, H552, S548, and S557, that were absent in the alleles found in all isolates with MICs of ≤1 mg/liter. Rifampin-susceptible isolates could be defined as those with MICs of ≤1 mg/liter. The rpoB allele sequence and isolate data have been incorporated into the PubMLST Neisseria database (http://pubmlst.org/neisseria/). The rifampin-resistant isolates belonged to diverse genetic lineages and were associated with lower levels of bacteremia and inflammatory cytokines in mice. This biological cost may explain the lack of clonal expansion of these isolates.

@article{taha_multicenter_2010, abstract = {Identification of clinical isolates of Neisseria meningitidis that are resistant to rifampin is important to avoid prophylaxis failure in contacts of patients, but it is hindered by the absence of a breakpoint for resistance, despite many efforts toward standardization. We examined a large number (n = 392) of clinical meningococcal isolates, spanning 25 years (1984 to 2009), that were collected in 11 European countries, Argentina, and the Central African Republic. The collection comprises all clinical isolates with {MICs} of [≥]0.25 mg/liter (n = 161) received by the national reference laboratories for meningococci in the participating countries. Representative isolates displaying rifampin {MICs} of {\textless}0.25 mg/liter were also examined (n = 231). Typing of isolates was performed, and a 660-bp {DNA} fragment of the {rpoB} gene was sequenced. Sequences differing by at least one nucleotide were defined as unique {rpoB} alleles. The geometric mean of the {MICs} was calculated for isolates displaying the same allele. The clinical isolates displaying rifampin {MICs} of {\textgreater}1 mg/liter possessed {rpoB} alleles with nonsynonymous mutations at four critical amino acid residues, D542, H552, S548, and S557, that were absent in the alleles found in all isolates with {MICs} of [≤]1 mg/liter. Rifampin-susceptible isolates could be defined as those with {MICs} of [≤]1 mg/liter. The {rpoB} allele sequence and isolate data have been incorporated into the {PubMLST} Neisseria database (http://pubmlst.org/neisseria/). The rifampin-resistant isolates belonged to diverse genetic lineages and were associated with lower levels of bacteremia and inflammatory cytokines in mice. This biological cost may explain the lack of clonal expansion of these isolates.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Taha, {Muhamed-Kheir} and Hedberg, Sara Thulin and Szatanik, Marek and Hong, Eva and Ruckly, Corinne and Abad, Raquel and Bertrand, Sophie and Carion, Francoise and Claus, Heike and Corso, Alejandra and Enriquez, Rocio and Heuberger, Sigrid and Hryniewicz, Waleria and Jolley, Keith A. and Kriz, Paula and Mollerach, Marta and Musilek, Martin and Neri, Arianna and Olcen, Per and Pana, Marina and Skoczynska, Anna and Pereira, Cecilia Sorhouet and Stefanelli, Paola and Tzanakaki, Georgina and Unemo, Magnus and Vazquez, Julio A. and Vogel, Ulrich and Wasko, Izabela}, biburl = {https://www.bibsonomy.org/bibtex/25e9c0e5b67a77c1c0bb47bb8ebbe08a9/jelias}, doi = {10.1128/AAC.00315-10}, interhash = {c37302605c02e35c8bceb534bb95844c}, intrahash = {5e9c0e5b67a77c1c0bb47bb8ebbe08a9}, journal = {Antimicrob. Agents Chemother.}, keywords = {imported}, month = sep, number = 9, pages = {3651--3658}, timestamp = {2011-03-11T10:05:40.000+0100}, title = {Multicenter Study for Defining the Breakpoint for Rifampin Resistance in Neisseria meningitidis by {rpoB} Sequencing}, url = {http://aac.asm.org/cgi/content/abstract/54/9/3651}, volume = 54, year = 2010 }