%0 Journal Article %1 lichter2022ultrastructural %A Lichter, Katharina %A Paul, Mila Marie %A Pauli, Martin %A Schoch, Susanne %A Kollmannsberger, Philip %A Stigloher, Christian %A Heckmann, Manfred %A Sirén, Anna-Leena %B Cell Reports %D 2022 %I Elsevier %J Cell Reports %K myown stigloher_high %N 12 %R 10.1016/j.celrep.2022.111382 %T Ultrastructural analysis of wild-type and RIM1α knockout active zones in a large cortical synapse %U https://doi.org/10.1016/j.celrep.2022.111382 %V 40 %X Rab3A-interacting molecule (RIM) is crucial for fast Ca2+-triggered synaptic vesicle (SV) release in presynaptic active zones (AZs). We investigated hippocampal giant mossy fiber bouton (MFB) AZ architecture in 3D using electron tomography of rapid cryo-immobilized acute brain slices in RIM1α?/? and wild-type mice. In RIM1α?/?, AZs are larger with increased synaptic cleft widths and a 3-fold reduced number of tightly docked SVs (0?2 nm). The distance of tightly docked SVs to the AZ center is increased from 110 to 195 nm, and the width of their electron-dense material between outer SV membrane and AZ membrane is reduced. Furthermore, the SV pool in RIM1α?/? is more heterogeneous. Thus, RIM1α, besides its role in tight SV docking, is crucial for synaptic architecture and vesicle pool organization in MFBs.

@article{lichter2022ultrastructural, abstract = {Rab3A-interacting molecule (RIM) is crucial for fast Ca2+-triggered synaptic vesicle (SV) release in presynaptic active zones (AZs). We investigated hippocampal giant mossy fiber bouton (MFB) AZ architecture in 3D using electron tomography of rapid cryo-immobilized acute brain slices in RIM1α?/? and wild-type mice. In RIM1α?/?, AZs are larger with increased synaptic cleft widths and a 3-fold reduced number of tightly docked SVs (0?2 nm). The distance of tightly docked SVs to the AZ center is increased from 110 to 195 nm, and the width of their electron-dense material between outer SV membrane and AZ membrane is reduced. Furthermore, the SV pool in RIM1α?/? is more heterogeneous. Thus, RIM1α, besides its role in tight SV docking, is crucial for synaptic architecture and vesicle pool organization in MFBs.}, added-at = {2022-09-26T08:34:36.000+0200}, author = {Lichter, Katharina and Paul, Mila Marie and Pauli, Martin and Schoch, Susanne and Kollmannsberger, Philip and Stigloher, Christian and Heckmann, Manfred and Sirén, Anna-Leena}, biburl = {https://www.bibsonomy.org/bibtex/236ec667e901186c7bb680384fa9edcf8/zam}, booktitle = {Cell Reports}, comment = {doi: 10.1016/j.celrep.2022.111382}, description = {Ultrastructural analysis of wild-type and RIM1α knockout active zones in a large cortical synapse: Cell Reports}, doi = {10.1016/j.celrep.2022.111382}, interhash = {c62c94ba668f026094fdf500cc3b6419}, intrahash = {36ec667e901186c7bb680384fa9edcf8}, issn = {22111247}, journal = {Cell Reports}, keywords = {myown stigloher_high}, month = sep, number = 12, publisher = {Elsevier}, timestamp = {2022-09-26T08:34:36.000+0200}, title = {Ultrastructural analysis of wild-type and RIM1α knockout active zones in a large cortical synapse}, url = {https://doi.org/10.1016/j.celrep.2022.111382}, volume = 40, year = 2022 }