%0 Journal Article %1 Berth1996a %A Berth, G. %A Dautzenberg, H. %A Christensen, B. E. %A Rother, G. %A Smidsrod, O. %C 605 THIRD AVE, NEW YORK, NY 10158-0012 %D 1996 %I JOHN WILEY & SONS INC %J Biopolymers %K ;; [ISI:] acetobacter-xylinum; alginate; aqueous chain dilute-solution; gpc polymers; polysaccharide; sodium-chloride; stiffness; xanthan; %N 5 %P 709 -- 719 %T Physicochemical studies on xylinan (acetan) .1. Characterization by gel permeation chromatography on sepharose Cl-2B coupled with static light scattering and viscometry %V 39 %X Laboratory-made samples of the polysaccharide xylinan (acetan) were fractionated on Sepharose Cl-2B using 0.1M NaCl as eluant. The weight average molar masses and intrinsic viscosities were estimated in the fractions by multiangle laser light scattering (off-line) and capillary viscometry, respectively. The Mauk-Houwink-Sakurada plot was found to be indicative of semiflexible coils (a = 0.90). The angular dependence of scattered Eight was interpreted by fitting with theoretically calculated ''Master Curves'' in terms of a wormlike chain model. The ambiguity of the interpretation of scattering curves owing to the overlapping effects of chain stiffness and polydispersity is discussed in detail The experimental data is found to be consistent with a persistence length of L(p) = 100 nm. The main proportion consists of double-stranded chains (consistent with a robust double-helix), but single- and multistranded chains also are present. Our results suggest a fractionation according to the contour length rather than the molar mass. (C) 1996 John Wiley & Sons, Inc.

@article{Berth1996a, __markedentry = {[phpts:6]}, abstract = {Laboratory-made samples of the polysaccharide xylinan (acetan) were fractionated on Sepharose Cl-2B using 0.1M NaCl as eluant. The weight average molar masses and intrinsic viscosities were estimated in the fractions by multiangle laser light scattering (off-line) and capillary viscometry, respectively. The Mauk-Houwink-Sakurada plot was found to be indicative of semiflexible coils (a = 0.90). The angular dependence of scattered Eight was interpreted by fitting with theoretically calculated ''Master Curves'' in terms of a wormlike chain model. The ambiguity of the interpretation of scattering curves owing to the overlapping effects of chain stiffness and polydispersity is discussed in detail The experimental data is found to be consistent with a persistence length of L(p) = 100 nm. The main proportion consists of double-stranded chains (consistent with a robust double-helix), but single- and multistranded chains also are present. Our results suggest a fractionation according to the contour length rather than the molar mass. (C) 1996 John Wiley & Sons, Inc.}, added-at = {2011-11-04T13:47:04.000+0100}, address = {605 THIRD AVE, NEW YORK, NY 10158-0012}, author = {Berth, G. and Dautzenberg, H. and Christensen, B. E. and Rother, G. and Smidsrod, O.}, authoraddress = {UNIV TRONDHEIM,NTH,DEPT BIOTECHNOL,NORWEGIAN BIOPOLYMER LAB,N-7034 TRONDHEIM,NORWAY.}, biburl = {https://www.bibsonomy.org/bibtex/273aba670ea1f989ba08f3f89104c1129/pawelsikorski}, citedref = {ATKINS EDT, 1990, NOVEL BIODEGRADABLE ; ATKINS EDT, 1994, 1 INT C POLYS ENG TR ; BERTH G, 1990, CARBOHYD POLYM, V12, P39 ; BERTH G, 1992, CARBOHYD POLYM, V19, P1 ; BERTH G, 1994, CARBOHYD POLYM, V25, P187 ; CHRISTENSEN BE, 1993, MACROMOLECULES, V26, P6111 ; COUSO RO, 1987, J GEN MICROBIOL, V133, P2123 ; DAUTZENBERG H, 1988, J POLYM SCI POL LETT, V26, P353 ; DAUTZENBERG H, 1992, MAKROMOL CHEM-M SYMP, V16, P94 ; DAUTZENBERG H, 1994, POLYELECTROLYTES FOR, CH5 ; DENKINGER P, 1991, J POLYM SCI POL PHYS, V29, P589 ; HARDING SE, 1991, ADV CARBOHYDRATE ANA, V1 ; HOLTZER AM, 1955, J POLYM SCI, V17, P432 ; HUGLIN MB, 1972, LIGHT SCATTERING POL ; JANSSON PE, 1993, CARBOHYD RES, V245, P303 ; KOYAMA R, 1973, J PHYS SOC JPN, V34, P1029 ; LIU W, 1987, CARBOHYD RES, V160, P267 ; MILLANE RP, 1992, GUMS STABILIZERS FOO, V6 ; MORRIS VJ, 1989, INT J BIOL MACROMOL, V11, P326 ; NORISUYE T, 1993, PROG POLYM SCI, V18, P543 ; PARADOSSI G, 1982, MACROMOLECULES, V15, P874 ; SATO T, 1984, MACROMOLECULES, V17, P2696 ; SCHMIDT M, 1985, MAKROMOL CHEM-RAPID, V6, P767 ; STOKKE BT, 1987, CARBOHYD RES, V160, P13 ; TANFORD C, 1965, PHYSICAL CHEM MACROM ; TAYAMA K, 1985, AGR BIOL CHEM TOKYO, V49, P959 ; TIDOUT MJ, 1994, INT J BIOL MACROMOL, V16, P324 ; VALLA S, 1981, CAN J MICROBIOL, V27, P599 ; WENG E, 1983, THESIS U TRONDHEIM N}, interhash = {c80f85a404cdf6cba5ac52cdfd7fb8fc}, intrahash = {73aba670ea1f989ba08f3f89104c1129}, isifile-dt = {Article}, isifile-ga = {VN232}, isifile-j9 = {BIOPOLYMERS}, isifile-nr = {29}, isifile-pi = {NEW YORK}, isifile-rp = {Berth, G, CARE OF DAUTZENBERG H,MPI COLLOID & INTERFACE RES,KANTSTR ; 55,D-14513 TELTOW,GERMANY.}, isifile-sc = {Biochemistry & Molecular Biology; Biophysics}, isifile-tc = {6}, issn = {0006-3525}, journal = {Biopolymers}, keywords = {;; [ISI:] acetobacter-xylinum; alginate; aqueous chain dilute-solution; gpc polymers; polysaccharide; sodium-chloride; stiffness; xanthan;}, language = {English}, month = NOV, number = 5, owner = {phpts}, pages = {709 -- 719}, publisher = {JOHN WILEY \& SONS INC}, size = {11 p.}, sourceid = {ISI:A1996VN23200009}, timestamp = {2011-11-04T13:47:06.000+0100}, title = {Physicochemical studies on xylinan (acetan) .1. Characterization by gel permeation chromatography on sepharose Cl-2B coupled with static light scattering and viscometry}, volume = 39, year = 1996 }