%0 Journal Article %1 Bunemann1995 %A Bunemann, M. %A Brandts, B. %A zu Heringdorf, D. M. %A van Koppen, C. J. %A Jakobs, K. H. %A Pott, L. %D 1995 %J J Physiol %K & *Lysophospholipids Acetylcholine/pharmacology Adenosine/pharmacology Agents/pharmacology Agonists/*pharmacology Animals Biotransformation/drug Cardiovascular Channels/drug Female GTP-Binding Guinea Heart/drug Male Membrane Muscarinic Myocardium/cytology/*metabolism Patch-Clamp Phospholipids/blood Pigs Potassium Potentials/drug Proteins/metabolism Signal Sphingosine/*analogs Techniques Transduction/drug derivatives/pharmacology effects effects/*metabolism effects/physiology %P 701-7 %T Activation of muscarinic K+ current in guinea-pig atrial myocytes by sphingosine-1-phosphate %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8788935 %V 489 ( Pt 3) %X 1. Activation of muscarinic K+ current (IK(ACh)) by sphingosine-1-phosphate (Sph-1-P) was studied in isolated cultured guinea-pig atrial myocytes using whole-cell voltage clamp. 2. Sph-1-P caused activation of IK(ACh) with an EC50 of 1.2 nM. The maximal current that could be activated by Sph-1-P amounted to about 90% of the IK(ACh) caused by a saturating concentration of acetylcholine (ACh, 10 microM). Sphingosine (1 microM), which can mimic the signalling effects of Sph-1-P in other cells, failed to cause measurable activation of IK(ACh). 3. IK(ACh) activation by Sph-1-P was completely suppressed in cells treated with pertussis toxin. 4. Desensitization of muscarinic receptors by pre-incubation of the cells with carbachol did not affect the response to Sph-1-P; likewise, pre-incubation of the cells with Sph-1-P resulted in a reduced sensitivity to the phospholipid but not to ACh. In contrast, pre-incubation with either Sph-1-P or a serum phospholipid previously described as activating atrial IK(ACh) resulted in reduced sensitivity to both phospholipids. 5. It is concluded that activation of IK(ACh) by Sph-1-P in atrial myocytes is induced by binding to a novel G protein-coupled phospholipid receptor.

@article{Bunemann1995, abstract = {1. Activation of muscarinic K+ current (IK(ACh)) by sphingosine-1-phosphate (Sph-1-P) was studied in isolated cultured guinea-pig atrial myocytes using whole-cell voltage clamp. 2. Sph-1-P caused activation of IK(ACh) with an EC50 of 1.2 nM. The maximal current that could be activated by Sph-1-P amounted to about 90% of the IK(ACh) caused by a saturating concentration of acetylcholine (ACh, 10 microM). Sphingosine (1 microM), which can mimic the signalling effects of Sph-1-P in other cells, failed to cause measurable activation of IK(ACh). 3. IK(ACh) activation by Sph-1-P was completely suppressed in cells treated with pertussis toxin. 4. Desensitization of muscarinic receptors by pre-incubation of the cells with carbachol did not affect the response to Sph-1-P; likewise, pre-incubation of the cells with Sph-1-P resulted in a reduced sensitivity to the phospholipid but not to ACh. In contrast, pre-incubation with either Sph-1-P or a serum phospholipid previously described as activating atrial IK(ACh) resulted in reduced sensitivity to both phospholipids. 5. It is concluded that activation of IK(ACh) by Sph-1-P in atrial myocytes is induced by binding to a novel G protein-coupled phospholipid receptor.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Bunemann, M. and Brandts, B. and zu Heringdorf, D. M. and van Koppen, C. J. and Jakobs, K. H. and Pott, L.}, biburl = {https://www.bibsonomy.org/bibtex/2943744138da616404cc303502b7aee61/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {d2e035b969d177083cc87c5c9e339b7f}, intrahash = {943744138da616404cc303502b7aee61}, issn = {0022-3751 (Print) 0022-3751 (Linking)}, journal = {J Physiol}, keywords = {& *Lysophospholipids Acetylcholine/pharmacology Adenosine/pharmacology Agents/pharmacology Agonists/*pharmacology Animals Biotransformation/drug Cardiovascular Channels/drug Female GTP-Binding Guinea Heart/drug Male Membrane Muscarinic Myocardium/cytology/*metabolism Patch-Clamp Phospholipids/blood Pigs Potassium Potentials/drug Proteins/metabolism Signal Sphingosine/*analogs Techniques Transduction/drug derivatives/pharmacology effects effects/*metabolism effects/physiology}, month = {Dec 15}, note = {Bunemann, M Brandts, B zu Heringdorf, D M van Koppen, C J Jakobs, K H Pott, L In Vitro Research Support, Non-U.S. Gov't England The Journal of physiology J Physiol. 1995 Dec 15;489 ( Pt 3):701-7.}, pages = {701-7}, shorttitle = {Activation of muscarinic K+ current in guinea-pig atrial myocytes by sphingosine-1-phosphate}, timestamp = {2010-12-14T18:12:06.000+0100}, title = {Activation of muscarinic K+ current in guinea-pig atrial myocytes by sphingosine-1-phosphate}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8788935}, volume = {489 ( Pt 3)}, year = 1995 }