%0 Journal Article %1 Deußner-Helfmann2018 %A Deußner-Helfmann, Nina S. %A Auer, Alexander %A Strauss, Maximilian T. %A Malkusch, Sebastian %A Dietz, Marina S. %A Barth, Hans-Dieter Dieter %A Jungmann, Ralf %A Heilemann, Mike %D 2018 %I American Chemical Society %J Nano Letters %K dna-origami fret microscopy multicolor multiplexing paint smlm superresolution %N 7 %P 4626--4630 %R 10.1021/acs.nanolett.8b02185 %T Correlative Single-Molecule FRET and DNA-PAINT Imaging %U http://pubs.acs.org/doi/10.1021/acs.nanolett.8b02185 https://pubs.acs.org/sharingguidelines %V 18 %X DNA-PAINT is an optical super-resolution microscopy method that can visualize nanoscale protein arrangements and provide spectrally unlimited multiplexing capabilities. However, current multiplexing implementations based on, for example, DNA exchange (such as Exchange-PAINT) achieves multitarget detection by sequential imaging, limiting throughput. Here, we combine DNA-PAINT with single-molecule FRET and use the FRET efficiency as parameter for multiplexed imaging with high specificity. We demonstrate correlated single-molecule FRET and super-resolution on DNA origami structures, which are equipped with binding sequences that are targeted by pairs of dye-labeled oligonucleotides generating the FRET signal. We futher extract FRET values from single binding sites that are spaced just ∼55 nm apart, demonstrating super-resolution FRET imaging. This combination of FRET and DNA-PAINT allows for multiplexed super-resolution imaging with low background and opens the door for accurate distance readout in the 1–10 ...

@article{Deußner-Helfmann2018, abstract = {DNA-PAINT is an optical super-resolution microscopy method that can visualize nanoscale protein arrangements and provide spectrally unlimited multiplexing capabilities. However, current multiplexing implementations based on, for example, DNA exchange (such as Exchange-PAINT) achieves multitarget detection by sequential imaging, limiting throughput. Here, we combine DNA-PAINT with single-molecule FRET and use the FRET efficiency as parameter for multiplexed imaging with high specificity. We demonstrate correlated single-molecule FRET and super-resolution on DNA origami structures, which are equipped with binding sequences that are targeted by pairs of dye-labeled oligonucleotides generating the FRET signal. We futher extract FRET values from single binding sites that are spaced just ∼55 nm apart, demonstrating super-resolution FRET imaging. This combination of FRET and DNA-PAINT allows for multiplexed super-resolution imaging with low background and opens the door for accurate distance readout in the 1–10 ...}, added-at = {2020-03-23T21:12:34.000+0100}, author = {Deu{\ss}ner-Helfmann, Nina S. and Auer, Alexander and Strauss, Maximilian T. and Malkusch, Sebastian and Dietz, Marina S. and Barth, Hans-Dieter Dieter and Jungmann, Ralf and Heilemann, Mike}, biburl = {https://www.bibsonomy.org/bibtex/226e93be44dcc0fdfd02b17baa551c69e/kfriedl}, doi = {10.1021/acs.nanolett.8b02185}, file = {:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Deu{\ss}ner-Helfmann et al. - 2018 - Correlative Single-Molecule FRET and DNA-PAINT Imaging.pdf:pdf}, interhash = {d6ce72005070b1aa796f9e5d0c370cb6}, intrahash = {26e93be44dcc0fdfd02b17baa551c69e}, issn = {15306992}, journal = {Nano Letters}, keywords = {dna-origami fret microscopy multicolor multiplexing paint smlm superresolution}, month = jul, number = 7, pages = {4626--4630}, pmid = {29943993}, publisher = {American Chemical Society}, timestamp = {2020-04-06T15:32:08.000+0200}, title = {{Correlative Single-Molecule FRET and DNA-PAINT Imaging}}, url = {http://pubs.acs.org/doi/10.1021/acs.nanolett.8b02185 https://pubs.acs.org/sharingguidelines}, volume = 18, year = 2018 }