Abnormalities of striatal dopaminergic neurotransmission play a significant role in the pathophysiology of central nervous system disorders such as movement disorders, addictions and schizophrenia. The striatum appears to be exposed to intrinsically high levels of oxidative stress (OS). Little is known, however, on the effect of OS on the regulation of the dopamine D2 receptor (DRD2), a key component of striatal dopaminergic neurotransmission. We report here on the effects of H2O2 (a canonical oxidant and non conventional messenger) and polyinosinic-polycytidylic acid (poly(IC) which elicits schizophrenia-like behaviors in newborn rodents and disrupts dopaminergic system development), on DRD2 levels in retinoic acid differentiated SH-SY5Y neuroblastoma. H2O2 elicited a significant increase in DRD2 mRNA and protein levels. Conversely, poly(IC) did not regulate DRD2 levels, although SH-SY5Y cells were confirmed to express TLR3 receptors. Under our conditions, H2O2, but not poly(IC), increased NFkappaB activation (as assessed by p65 nuclear translocation), which paralleled their effects on DRD2 levels regulation.
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%0 Journal Article
%1 larouche_subacute_2008
%A Larouche, Annie
%A Berube, Patrick
%A Sarret, Philippe
%A Grignon, Sylvain
%D 2008
%J Synapse (New York, N.Y.)
%K Blotting Cell_Differentiation Cell_Line Dopamine_D2 Electrophoresis Gene_Expression Gene_Expression_Regulation Humans Hydrogen_Peroxide Interferon_Inducers Messenger Neurons Oxidants Oxidative_Stress Poly_{I-C} Polyacrylamide_Gel Polymerase_Chain_Reaction Receptors Tretinoin Tumor Western {NF-kappa}_B {RNA}
%N 1
%P 70--73
%R 10.1002/syn.20458
%T Subacute H2O2, but not poly(IC), upregulates dopamine D2 receptors in retinoic acid differentiated SH-SY5Y neuroblastoma
%U http://www.ncbi.nlm.nih.gov/pubmed/17960767
%V 62
%X Abnormalities of striatal dopaminergic neurotransmission play a significant role in the pathophysiology of central nervous system disorders such as movement disorders, addictions and schizophrenia. The striatum appears to be exposed to intrinsically high levels of oxidative stress (OS). Little is known, however, on the effect of OS on the regulation of the dopamine D2 receptor (DRD2), a key component of striatal dopaminergic neurotransmission. We report here on the effects of H2O2 (a canonical oxidant and non conventional messenger) and polyinosinic-polycytidylic acid (poly(IC) which elicits schizophrenia-like behaviors in newborn rodents and disrupts dopaminergic system development), on DRD2 levels in retinoic acid differentiated SH-SY5Y neuroblastoma. H2O2 elicited a significant increase in DRD2 mRNA and protein levels. Conversely, poly(IC) did not regulate DRD2 levels, although SH-SY5Y cells were confirmed to express TLR3 receptors. Under our conditions, H2O2, but not poly(IC), increased NFkappaB activation (as assessed by p65 nuclear translocation), which paralleled their effects on DRD2 levels regulation.
@article{larouche_subacute_2008,
abstract = {Abnormalities of striatal dopaminergic neurotransmission play a significant role in the pathophysiology of central nervous system disorders such as movement disorders, addictions and schizophrenia. The striatum appears to be exposed to intrinsically high levels of oxidative stress {(OS).} Little is known, however, on the effect of {OS} on the regulation of the dopamine D2 receptor {(DRD2)}, a key component of striatal dopaminergic neurotransmission. We report here on the effects of {H2O2} (a canonical oxidant and non conventional messenger) and polyinosinic-polycytidylic acid {(poly(IC)} which elicits schizophrenia-like behaviors in newborn rodents and disrupts dopaminergic system development), on {DRD2} levels in retinoic acid differentiated {SH-SY5Y} neuroblastoma. {H2O2} elicited a significant increase in {DRD2} {mRNA} and protein levels. Conversely, {poly(IC)} did not regulate {DRD2} levels, although {SH-SY5Y} cells were confirmed to express {TLR3} receptors. Under our conditions, {H2O2}, but not {poly(IC)}, increased {NFkappaB} activation (as assessed by p65 nuclear translocation), which paralleled their effects on {DRD2} levels regulation.},
added-at = {2011-08-03T17:38:07.000+0200},
author = {Larouche, Annie and Berube, Patrick and Sarret, Philippe and Grignon, Sylvain},
biburl = {https://www.bibsonomy.org/bibtex/24005f0c1d595ac481b4b644ae7fbad23/crc_chus},
doi = {10.1002/syn.20458},
interhash = {ded1105a847fa68c5637466a01821549},
intrahash = {4005f0c1d595ac481b4b644ae7fbad23},
issn = {0887-4476},
journal = {Synapse {(New} York, {N.Y.)}},
keywords = {Blotting Cell_Differentiation Cell_Line Dopamine_D2 Electrophoresis Gene_Expression Gene_Expression_Regulation Humans Hydrogen_Peroxide Interferon_Inducers Messenger Neurons Oxidants Oxidative_Stress Poly_{I-C} Polyacrylamide_Gel Polymerase_Chain_Reaction Receptors Tretinoin Tumor Western {NF-kappa}_B {RNA}},
month = jan,
note = {{PMID:} 17960767},
number = 1,
pages = {70--73},
timestamp = {2011-08-03T20:52:18.000+0200},
title = {Subacute {H2O2}, but not {poly(IC)}, upregulates dopamine D2 receptors in retinoic acid differentiated {SH-SY5Y} neuroblastoma},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17960767},
volume = 62,
year = 2008
}