%0 Journal Article %1 citeulike:488368 %A Broyles, Celeste A. %A Rechnitz, Garry A. %C Department of Chemistry, University of Delaware, Newark, Delaware 19716 %D 1986 %J Analytical Chemistry %K displacement emit biosensor immunoassay homogeneous immunosensor ag_detection electrochemistry tagged_mediator %N 6 %P 1241--1245 %R 10.1021/ac00297a060 %T Drug antibody measurement by homogeneous enzyme immunoassay with amperometric detection. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3521389 %V 58 %X A homogeneous enzyme Immunoassay Is described for the determination of drug antlbodles. The method Is based upon inhlbitlon of the enzyme actlvity of an enzyme-antigen conjugate by the correspondlng antlbody that Is to be measured. The activity Is monitored by amperometrlc detection of fhe rate of NADH oxidation at a platinum electrode. The technique Is Illustrated by uslng the lldocalne-antl-lldocalne system and ylelds callbration curves at nanogram levels of antlbody with absolute sensltlvlty dependent upon the original amount of enzyme-antlgen conjugate. Interferences such as protein adsorption and antl-qulnidine cross-reactivity are shown to have mlnlmal effect on the assay, whlch has a precislon of 5%.

@article{citeulike:488368, abstract = {A homogeneous enzyme Immunoassay Is described for the determination of drug antlbodles. The method Is based upon inhlbitlon of the enzyme actlvity of an enzyme-antigen conjugate by the correspondlng antlbody that Is to be measured. The activity Is monitored by amperometrlc detection of fhe rate of NADH oxidation at a platinum electrode. The technique Is Illustrated by uslng the lldocalne-antl-lldocalne system and ylelds callbration curves at nanogram levels of antlbody with absolute sensltlvlty dependent upon the original amount of enzyme-antlgen conjugate. Interferences such as protein adsorption and antl-qulnidine cross-reactivity are shown to have mlnlmal effect on the assay, whlch has a precislon of 5%.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Department of Chemistry, University of Delaware, Newark, Delaware 19716}, author = {Broyles, Celeste A. and Rechnitz, Garry A.}, biburl = {https://www.bibsonomy.org/bibtex/2af9f661d7f7ec1ce00de4b1ecf6b81d6/biblio24}, citeulike-article-id = {488368}, doi = {10.1021/ac00297a060}, interhash = {e0f8a5b10d0e7d5e8c9b82dda592d9f8}, intrahash = {af9f661d7f7ec1ce00de4b1ecf6b81d6}, issn = {0003-2700}, journal = {Analytical Chemistry}, keywords = {displacement emit biosensor immunoassay homogeneous immunosensor ag_detection electrochemistry tagged_mediator}, month = May, number = 6, pages = {1241--1245}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Drug antibody measurement by homogeneous enzyme immunoassay with amperometric detection.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=3521389}, volume = 58, year = 1986 }