Article,
Adaptive Evolution Of Escherichia coli K-12 MG1655 On A Non-Native Carbon Source, L-1,2-Propanediol.
Applied and environmental microbiology, (Apr 30, 2010)
DOI: 10.1128/aem.00373-10
Abstract
Laboratory adaptive evolution can provide key information to address a wide range of issues in evolutionary biology. Such studies have been limited thus far by the inability to readily detect mutations on a genome-scale in evolved microbial strains. This limitation has now been overcome by recently-developed genome sequencing technology that readily allows us to identify all accumulated mutations that appear during laboratory adaptive evolution. In this study, we evolved Escherichia coli K-12 MG1655 on a non-native carbon source, L-1,2-propanediol (L-1,2-PDO) for approximately 700 generations. We found that; i) Experimental evolution of E. coli for approximately 700 generations in 1,2-PDO-supplemented minimal medium revealed the acquisition of the ability to use L-1,2-PDO as the sole carbon and energy source, from no growth at all initially, to a growth rate of 0.35 h(-1), ii) Six mutations detected by whole genome re-sequencing were accumulated in the evolved E. coli mutant over the course of adaptive evolution on L-1,2-PDO, iii) Five of six mutations were within coding regions, with IS5 insertion between two fuc regulons, iv) Two major mutations (mutations in fucO and its promoter) involved in L-1,2-PDO catabolism appeared early during adaptive evolution, v) Multiple defined knock-in mutant strains with all mutations had growth rates essentially matching that of the evolved strain. These results provide insight into the genetic basis underlying microbial evolution to growth on the non-native substrate.
