%0 Journal Article %1 Jeon2009 %A Jeon, Hojeong %A Lee, Yongku %A Jin, Songwan %A Koo, Sangmo %A Lee, Chang-Soo %A Yoo, JungYul %D 2009 %I Springer US %J Biomedical Microdevices %K bacteria chemotaxis microfluidics %N 5 %P 1135-1143 %R 10.1007/s10544-009-9330-8 %T Quantitative analysis of single bacterial chemotaxis using a linear concentration gradient microchannel %U http://dx.doi.org/10.1007/s10544-009-9330-8 %V 11 %X A microfluidic device to quantify bacterial chemotaxis has been proposed, which generates a linear concentration gradient of chemoattractant in the main channel only by convective and molecular diffusion, and which enables the bacteria to enter the main channel in a single file by hydrodynamic focusing technique. The trajectory of each bacterium in response to the concentration gradient of chemoattractant is photographed by a CCD camera and its velocity is acquired by a simple PTV (Particle Tracking Velocimetry) algorithm. An advantage of this assay is to measure the velocity of a single bacterium and to quantify the degree of chemotaxis by analyzing the frequency of velocities concurrently. Thus, the parameter characterizing the motility of wild-type

@article{Jeon2009, abstract = {A microfluidic device to quantify bacterial chemotaxis has been proposed, which generates a linear concentration gradient of chemoattractant in the main channel only by convective and molecular diffusion, and which enables the bacteria to enter the main channel in a single file by hydrodynamic focusing technique. The trajectory of each bacterium in response to the concentration gradient of chemoattractant is photographed by a CCD camera and its velocity is acquired by a simple PTV (Particle Tracking Velocimetry) algorithm. An advantage of this assay is to measure the velocity of a single bacterium and to quantify the degree of chemotaxis by analyzing the frequency of velocities concurrently. Thus, the parameter characterizing the motility of wild-type }, added-at = {2013-12-20T12:48:35.000+0100}, author = {Jeon, Hojeong and Lee, Yongku and Jin, Songwan and Koo, Sangmo and Lee, Chang-Soo and Yoo, JungYul}, biburl = {https://www.bibsonomy.org/bibtex/24f57e16acc497712229164b4a25497ec/quantentunnel}, doi = {10.1007/s10544-009-9330-8}, interhash = {e9e21a8074d9738f9fe330644533d2de}, intrahash = {4f57e16acc497712229164b4a25497ec}, issn = {1387-2176}, journal = {Biomedical Microdevices}, keywords = {bacteria chemotaxis microfluidics}, language = {English}, number = 5, pages = {1135-1143}, publisher = {Springer US}, timestamp = {2013-12-20T12:48:35.000+0100}, title = {Quantitative analysis of single bacterial chemotaxis using a linear concentration gradient microchannel}, url = {http://dx.doi.org/10.1007/s10544-009-9330-8}, volume = 11, year = 2009 }