%0 Journal Article %1 Seefeldt2008 %A Seefeldt, Britta %A Kasper, Robert %A Seidel, Thorsten %A Tinnefeld, Philip %A Dietz, Karl-Josef %A Heilemann, Mike %A Sauer, Markus %C PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY %D 2008 %I WILEY-V C H VERLAG GMBH %J JOURNAL OF BIOPHOTONICS %K sauer mike %N 1 %P 74-82 %T Fluorescent proteins for single-molecule fluorescence applications %U http://dx.doi.org/10.1002/jbio.200710024 %V 1 %X We present single-molecule fluorescence data of fluorescent proteins GFP, YFP, DsRed, and mCherry, a new derivative of DsRed. Ensemble and single-molecule fluorescence experiments proved mCherry as an ideally suited fluorophore for single-molecule applications, demonstrated by high photostability and rare fluorescence-intensity fluctuations. Although mCherry exhibits the lowest fluorescence quantum yield among the fluorescent proteins investigated, its superior photophysical characteristics suggest mCherry as an ideal alternative in single-molecule fluorescence experiments. Due to its spectral characteristics and short fluorescence lifetime of 1.46 ns, mCherry complements other existing fluorescent proteins and is recommended for tracking and localization of target molecules with high accuracy, fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging microscopy (FLIM), or multicolor applications. GRAPHICS mCherry exhibits a relatively high photostability and brightness under single-molecule fluorescence imaging conditions. (C) 2008 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

@article{Seefeldt2008, abstract = {We present single-molecule fluorescence data of fluorescent proteins GFP, YFP, DsRed, and mCherry, a new derivative of DsRed. Ensemble and single-molecule fluorescence experiments proved mCherry as an ideally suited fluorophore for single-molecule applications, demonstrated by high photostability and rare fluorescence-intensity fluctuations. Although mCherry exhibits the lowest fluorescence quantum yield among the fluorescent proteins investigated, its superior photophysical characteristics suggest mCherry as an ideal alternative in single-molecule fluorescence experiments. Due to its spectral characteristics and short fluorescence lifetime of 1.46 ns, mCherry complements other existing fluorescent proteins and is recommended for tracking and localization of target molecules with high accuracy, fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging microscopy (FLIM), or multicolor applications. {[}GRAPHICS] mCherry exhibits a relatively high photostability and brightness under single-molecule fluorescence imaging conditions. (C) 2008 by WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim}, added-at = {2011-03-01T10:57:13.000+0100}, address = {PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY}, affiliation = {Heilemann, M (Reprint Author), Univ Bielefeld, Dept Phys, Univ Str 25, D-33615 Bielefeld, Germany. {[}Seefeldt, Britta; Kasper, Robert; Heilemann, Mike; Sauer, Markus] Univ Bielefeld, Dept Phys, D-33615 Bielefeld, Germany. {[}Seidel, Thorsten; Dietz, Karl-Josef] Univ Bielefeld, Dept Biochem \& Physiol Plants, D-33501 Bielefeld, Germany. {[}Tinnefeld, Philip] Univ Munich, Dept Appl Phys Biophys, D-80799 Munich, Germany. {[}Tinnefeld, Philip] Univ Munich, Ctr Nanosci, D-80799 Munich, Germany.}, author = {Seefeldt, Britta and Kasper, Robert and Seidel, Thorsten and Tinnefeld, Philip and Dietz, Karl-Josef and Heilemann, Mike and Sauer, Markus}, author-email = {heileman@physik.uni-bielefeld.de sauer@physik.uni-bielefeld.de}, biburl = {https://www.bibsonomy.org/bibtex/21fbeddf520dd77618d55355667e38fed/reichert}, cited-references = {ANDO R, 2004, SCIENCE, V306, P1370. 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WEISS S, 2005, SCIENCE 1999, V283, P1676. ZUMBUSCH A, 2000, SINGLE MOL, V1, P261.}, doc-delivery-number = {382SZ}, funding-acknowledgement = {German Research Foundation {[}SFB 613, A5]}, funding-text = {The authors are grateful to the German Research Foundation (SFB 613, A5) for funding.}, groups = {public}, interhash = {ec6a23dbafcf45aefd7ac12c2cd0845f}, intrahash = {1fbeddf520dd77618d55355667e38fed}, issn = {1864-063X}, journal = {JOURNAL OF BIOPHOTONICS}, journal-iso = {J. Biophotonics}, keywords = {sauer mike}, keywords-plus = {AUTOFLUORESCENT PROTEINS; SPECTROSCOPY; CELLS; GFP; TRACKING; MICROSCOPY; DRONPA; RED}, language = {English}, month = FEB, number = 1, number-of-cited-references = {34}, pages = {74-82}, publisher = {WILEY-V C H VERLAG GMBH}, subject-category = {Biochemical Research Methods; Biophysics}, times-cited = {11}, timestamp = {2011-03-09T14:16:00.000+0100}, title = {Fluorescent proteins for single-molecule fluorescence applications}, type = {Article}, unique-id = {ISI:000261627000017}, url = {http://dx.doi.org/10.1002/jbio.200710024}, username = {reichert}, volume = 1, year = 2008 }