%0 Journal Article %1 markakukuckahpl %A Kukucka, Mark A. %A Misra, Hara P. %D 1992 %J Arch Androl %K Leydig cells oxytocin %N 2 %P 185-190 %R 10.3109/01485019208987723 %T HPLC determination of an oxytocin-like peptide produced by isolated guinea pig Leydig cells: stimulation by ascorbate. %U http://www.hubmed.org/tags/item/1456839 %V 29 %X Highly purified populations of guinea pig Leydig cells were incubated with a maximally stimulating dose of 100 ng/mL LH for 24 h in the presence of increasing concentrations of sodium ascorbate. Sample supernatants were extracted, concentrated under vacuum, and reconstituted with acidified absolute ethanol. Samples were analyzed for oxytocin using high-performance liquid chromatography with electrochemical detection and known concentrations of an authentic oxytocin standard. Leydig cells stimulated with 0, 25, and 50 microM ascorbate produced and secreted 40.1 +/- 1.23, 77.4 +/- 13.8, 74.2 +/- 26.3 pg of an oxytocin-like peptide, respectively, per 1 x 10(6) cells. These results indicate that guinea pig Leydig cells are capable of producing an oxytocin-like peptide de novo and that low concentrations of ascorbate stimulate the production of this peptide in Leydig cells cultured in vitro.

@article{markakukuckahpl, abstract = {Highly purified populations of guinea pig Leydig cells were incubated with a maximally stimulating dose of 100 ng/mL LH for 24 h in the presence of increasing concentrations of sodium ascorbate. Sample supernatants were extracted, concentrated under vacuum, and reconstituted with acidified absolute ethanol. Samples were analyzed for oxytocin using high-performance liquid chromatography with electrochemical detection and known concentrations of an authentic oxytocin standard. Leydig cells stimulated with 0, 25, and 50 microM ascorbate produced and secreted 40.1 +/- 1.23, 77.4 +/- 13.8, 74.2 +/- 26.3 pg of an oxytocin-like peptide, respectively, per 1 x 10(6) cells. These results indicate that guinea pig Leydig cells are capable of producing an oxytocin-like peptide de novo and that low concentrations of ascorbate stimulate the production of this peptide in Leydig cells cultured in vitro.}, added-at = {2010-02-13T05:04:38.000+0100}, author = {Kukucka, Mark A. and Misra, Hara P.}, biburl = {https://www.bibsonomy.org/bibtex/2bd10c02ea6c5b301734bf87d51e750be/dvmphd}, doi = {10.3109/01485019208987723}, interhash = {ec6b4a183faf08261a500087a49fd7c6}, intrahash = {bd10c02ea6c5b301734bf87d51e750be}, journal = {Arch Androl}, keywords = {Leydig cells oxytocin}, note = {PMID: 1456839 [PubMed - indexed for MEDLINE] n.b. This study was presented in part at the Third Research Day Proceedings held at the Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia, May 23-24, 1991. The published proceedings (No. 23919419) were catalogued with the Online Computer Library Center (OCLC) database on June 11, 1991. Hence, this is the very first report in the scientific literature which conclusively demonstrates that Leydig cells are capable of producing testicular oxytocin de novo.}, number = 2, pages = {185-190}, timestamp = {2012-05-10T15:16:02.000+0200}, title = {HPLC determination of an oxytocin-like peptide produced by isolated guinea pig Leydig cells: stimulation by ascorbate.}, url = {http://www.hubmed.org/tags/item/1456839}, volume = 29, year = 1992 }