%0 Journal Article %1 Xiang2007 %A Xiang, Q. %A Xu, B. %A Li, D. %D 2007 %J BIOMEDICAL MICRODEVICES %K iontek %N 4 %P 443-449 %R 10.1007/s10544-007-9048-4 %T Miniature real time PCR on chip with multi-channel fiber optical fluorescence detection module %V 9 %X This paper presents the design and implementation of a miniature real time PCR system consisting of a disposable reactor chip, a miniature thermal cycler, and a multi-channel fiber optical fluorescence excitation/detection module. The disposable PCR chip is fabricated by using soft photolithography by PDMS (Polydimethylsiloxane) and glass. The miniature thermal cycler has a thin film heater for heating and a fan for rapid cooling. The fiber optical detection module consists of laser, filter cube, photo-detector and 1x4 fiber optical switch. It is capable of four-well real time PCR analysis. Real-time PCR detection of E. coli stx1 has been demonstrated successfully with this system.

@article{Xiang2007, abstract = {{This paper presents the design and implementation of a miniature real time PCR system consisting of a disposable reactor chip, a miniature thermal cycler, and a multi-channel fiber optical fluorescence excitation/detection module. The disposable PCR chip is fabricated by using soft photolithography by PDMS (Polydimethylsiloxane) and glass. The miniature thermal cycler has a thin film heater for heating and a fan for rapid cooling. The fiber optical detection module consists of laser, filter cube, photo-detector and 1x4 fiber optical switch. It is capable of four-well real time PCR analysis. Real-time PCR detection of E. coli stx1 has been demonstrated successfully with this system.}}, added-at = {2011-01-19T09:25:00.000+0100}, affiliation = {Li, D (Reprint Author), Vanderbilt Univ, Dept Mech Engn, 2301 Vanderbilt Pl, Nashville, TN 37235 USA. Vanderbilt Univ, Dept Mech Engn, Nashville, TN 37235 USA. Univ Toronto, Dept Mech \& Ind Engn, Toronto, ON M5S 3G8, Canada.}, author = {Xiang, Q. and Xu, B. and Li, D.}, biburl = {https://www.bibsonomy.org/bibtex/2e6745dec08220a655501ebcd432c8f0d/hkayabilisim}, category = {Engineering, Biomedical; Nanoscience \& Nanotechnology}, cited = {12}, doi = {10.1007/s10544-007-9048-4}, email = {dongqing.li@vanderbilt.edu}, file = {:home/hkaya/Projeler/Robot/doc/literature/Xiang2007.pdf:PDF}, id = {ISI:000248007500003}, interhash = {ec6c30fbcf9b05b840e090a16b0f52c3}, intrahash = {e6745dec08220a655501ebcd432c8f0d}, iso = {Biomed. Microdevices}, issn = {1387-2176}, journal = {{BIOMEDICAL MICRODEVICES}}, keywords = {iontek}, language = {English}, month = {{AUG}}, number = {{4}}, pages = {{443-449}}, references = {25}, timestamp = {2011-01-19T09:25:00.000+0100}, title = {{Miniature real time PCR on chip with multi-channel fiber optical fluorescence detection module}}, type = {{Article}}, volume = {{9}}, year = {{2007}} }