%0 Journal Article %1 citeulike:482289 %A Feldman, Sandra F. %A Uzgiris, Egidijus E. %A Y., John %A Gui, %A Shu, Emily Y. %A Stokes, Edward B. %D 1995 %J Biosensors and Bioelectronics %K methodology immunosensor statistic %N 5 %P 423--434 %R 10.1016/0956-5663(95)96889-7 %T Evanescent wave immunoprobe with high bivalent antibody activity %U http://www.sciencedirect.com/science/article/B6TFC-3XY2HTY-57/2/20ca3cb2f0cd02f2bf1e2f2e9ba0833d %V 10 %X We have determined the kinetic response of an evanescent wave optical fibre immunosensor and its absolute sensitivity. Using both kinetic methods and optical determinations of bound antigen, we infer that there are 2middle dot4 x 1011 active antibodies per cm2 probe area. We estimate that 75% of the active antibodies are in the bivalent form, with both binding site capable of binding antigen. Using the kinetic data and optical measurements of the number of antigens bound to the fibre, we estimate that the unstirred dead layer thickness next to the fibre surface in the stirred solution, across which the antigens must diffuse, is 55 mum. Approximately 109 FITC labelled antigens are necessary to achieve a threshold signal, defined as 1% of the signal obtained when the fibre is saturated with antigen.

@article{citeulike:482289, abstract = {We have determined the kinetic response of an evanescent wave optical fibre immunosensor and its absolute sensitivity. Using both kinetic methods and optical determinations of bound antigen, we infer that there are 2[middle dot]4 x 1011 active antibodies per cm2 probe area. We estimate that 75% of the active antibodies are in the bivalent form, with both binding site capable of binding antigen. Using the kinetic data and optical measurements of the number of antigens bound to the fibre, we estimate that the unstirred dead layer thickness next to the fibre surface in the stirred solution, across which the antigens must diffuse, is 55 [mu]m. Approximately 109 FITC labelled antigens are necessary to achieve a threshold signal, defined as 1% of the signal obtained when the fibre is saturated with antigen.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Feldman, Sandra F. and Uzgiris, Egidijus E. and Y., John and Gui and Shu, Emily Y. and Stokes, Edward B.}, biburl = {https://www.bibsonomy.org/bibtex/2add144424f8ef132fe1bf753e6b5f3c3/biblio24}, citeulike-article-id = {482289}, doi = {10.1016/0956-5663(95)96889-7}, interhash = {f196024eca3bfa0b7d9ef78133efd565}, intrahash = {add144424f8ef132fe1bf753e6b5f3c3}, journal = {Biosensors and Bioelectronics}, keywords = {methodology immunosensor statistic}, number = 5, pages = {423--434}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Evanescent wave immunoprobe with high bivalent antibody activity}, url = {http://www.sciencedirect.com/science/article/B6TFC-3XY2HTY-57/2/20ca3cb2f0cd02f2bf1e2f2e9ba0833d}, volume = 10, year = 1995 }