%0 Journal Article %1 Bret_2002_H1720 %A Brette, Fabien %A Komukai, Kimiaki %A Orchard, Clive H %D 2002 %J Am. J. Physiol. Heart Circ. Physiol. %K 12234828 Action Agents, Animals, Atria, Calcium, Cardiotonic Compounds, Dyes, Electrophysiology, Fibers, Fluorescent Formamides, Gov't, Heart Isoproterenol, Microtubules, Muscle Myocardium, Non-U.S. Potentials, Pyridinium Rats, Research Sarcolemma, Support, %N 4 %P H1720-8 %R 10.1152/ajpheart.00347.2002 %T Validation of formamide as a detubulation agent in isolated rat cardiac cells. %U http://dx.doi.org/10.1152/ajpheart.00347.2002 %V 283 %X Kawai M, Hussain M, and Orchard CH. Am J Heart Circ Physiol 277: H603-H609, 1999 developed a technique to detubulate rat ventricular myocytes using formamide and showed that detubulation results in a decrease in cell capacitance, Ca$^2+$ current density, and Ca$^2+$ transient amplitude. We have investigated the mechanism of this detubulation and possible direct effects of formamide. Staining ventricular cells with di-8-ANEPPS showed that the t tubule membranes remain inside the cell after detubulation; trapping of FITC-labeled dextran within the t tubules showed that detubulation occurs during formamide washout and that the t tubules appear to reseal within the cell. Detubulation had no effect on the microtubule network but resulted in loss of synchronous Ca$^2+$ release on electrical stimulation. In contrast, formamide treatment of atrial cells did not significantly change cell capacitance, Ca$^2+$ current amplitude, action potential configuration, the Ca$^2+$ transient or the response of the Ca$^2+$ transient to isoprenaline. We conclude that formamide washout induces detubulation of single rat ventricular myocytes, leaving the t tubules within the cell, but without direct effects on cell proteins that might alter cell function.

@article{Bret_2002_H1720, abstract = {Kawai M, Hussain M, and Orchard {CH}. Am J Heart Circ Physiol 277: H603-H609, 1999 developed a technique to detubulate rat ventricular myocytes using formamide and showed that detubulation results in a decrease in cell capacitance, {C}a$^{2+}$ current density, and {C}a$^{2+}$ transient amplitude. We have investigated the mechanism of this detubulation and possible direct effects of formamide. Staining ventricular cells with di-8-{ANEPPS} showed that the t tubule membranes remain inside the cell after detubulation; trapping of {FITC}-labeled dextran within the t tubules showed that detubulation occurs during formamide washout and that the t tubules appear to reseal within the cell. Detubulation had no effect on the microtubule network but resulted in loss of synchronous {C}a$^{2+}$ release on electrical stimulation. In contrast, formamide treatment of atrial cells did not significantly change cell capacitance, {C}a$^{2+}$ current amplitude, action potential configuration, the {C}a$^{2+}$ transient or the response of the {C}a$^{2+}$ transient to isoprenaline. We conclude that formamide washout induces detubulation of single rat ventricular myocytes, leaving the t tubules within the cell, but without direct effects on cell proteins that might alter cell function.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Brette, Fabien and Komukai, Kimiaki and Orchard, Clive H}, biburl = {https://www.bibsonomy.org/bibtex/273efefa83980333fb35b1996818b3493/hake}, description = {The whole bibliography file I use.}, doi = {10.1152/ajpheart.00347.2002}, file = {Bret_2002_H1720.pdf:Bret_2002_H1720.pdf:PDF}, interhash = {f425af2da98dd1a0096fce3a3e5bedb0}, intrahash = {73efefa83980333fb35b1996818b3493}, journal = {Am. J. Physiol. Heart Circ. Physiol.}, key = 40, keywords = {12234828 Action Agents, Animals, Atria, Calcium, Cardiotonic Compounds, Dyes, Electrophysiology, Fibers, Fluorescent Formamides, Gov't, Heart Isoproterenol, Microtubules, Muscle Myocardium, Non-U.S. Potentials, Pyridinium Rats, Research Sarcolemma, Support,}, month = Oct, number = 4, pages = {H1720-8}, pii = {00347.2002}, timestamp = {2009-06-03T11:21:06.000+0200}, title = {Validation of formamide as a detubulation agent in isolated rat cardiac cells.}, url = {http://dx.doi.org/10.1152/ajpheart.00347.2002}, volume = 283, year = 2002 }