%0 Journal Article %1 friedrich2023detection %A Friedrich, Bernhard %A Vogel, Patrick %A Rückert, Martin A. %A Lyer, Stefan %A Günther, Johanna %A Wernery, Ulrich %A Joseph, Sunitha %A Müller, Judith %A Behr, Volker C. %A Alexiou, Christoph %A Tietze, Rainer %D 2023 %J Appl. Microbiol. Biotechnol. %K mpi %N 10 %P 3329-3339 %R 10.1007/s00253-023-12513-4 %T Detection of viral antibodies in camel sera using magnetic particle spectroscopy %V 107 %X Pandemics like SARS-Cov-2 very frequently have their origin in different animals and in particular herds of camels could be a source of zoonotic diseases. This study took advantage on a highly sensitive and adaptable method for the fast and reliable detection of viral antibodies in camels using low-cost equipment. Magnetic nanoparticles (MNP) have high variability in their functionalization with different peptides and proteins. We confirm that 3-aminopropyl triethoxysilane (APTES)-coated MNP could be functionalized with viral proteins. The protein loading could be confirmed by simple loading controls using FACS-analysis (p < 0.05). Complementary combination of antigen and antibody yields in a significant signal increase could be proven by both FACS and COMPASS. However, COMPASS needs only a few seconds for the measurement. In COMPASS, the phase φn on selected critical point of the fifth higher harmonic (n = 5th). Here, positive sera display highly significant signal increase over the control or negative sera. Furthermore, a clear distinction could be made in antibody detection as an immune response to closely related viruses (SARS-CoV2 and MERS). Using modified MNPs along with COMPASS offers a fast and reliable method that is less cost intensive than current technologies and offers the possibility to be quickly adapted in case of new occurring viral infections.

@article{friedrich2023detection, abstract = {Pandemics like SARS-Cov-2 very frequently have their origin in different animals and in particular herds of camels could be a source of zoonotic diseases. This study took advantage on a highly sensitive and adaptable method for the fast and reliable detection of viral antibodies in camels using low-cost equipment. Magnetic nanoparticles (MNP) have high variability in their functionalization with different peptides and proteins. We confirm that 3-aminopropyl triethoxysilane (APTES)-coated MNP could be functionalized with viral proteins. The protein loading could be confirmed by simple loading controls using FACS-analysis (p < 0.05). Complementary combination of antigen and antibody yields in a significant signal increase could be proven by both FACS and COMPASS. However, COMPASS needs only a few seconds for the measurement. In COMPASS, the phase φn on selected critical point of the fifth higher harmonic (n = 5th). Here, positive sera display highly significant signal increase over the control or negative sera. Furthermore, a clear distinction could be made in antibody detection as an immune response to closely related viruses (SARS-CoV2 and MERS). Using modified MNPs along with COMPASS offers a fast and reliable method that is less cost intensive than current technologies and offers the possibility to be quickly adapted in case of new occurring viral infections.}, added-at = {2023-06-15T22:49:36.000+0200}, author = {Friedrich, Bernhard and Vogel, Patrick and Rückert, Martin A. and Lyer, Stefan and Günther, Johanna and Wernery, Ulrich and Joseph, Sunitha and Müller, Judith and Behr, Volker C. and Alexiou, Christoph and Tietze, Rainer}, biburl = {https://www.bibsonomy.org/bibtex/25ca4aa536983443f4c687b0219e109a6/ep5mpi}, doi = {10.1007/s00253-023-12513-4}, interhash = {f430acdda2a705d16c6795d04efe8090}, intrahash = {5ca4aa536983443f4c687b0219e109a6}, issn = {1432-0614}, journal = {Appl. Microbiol. Biotechnol.}, keywords = {mpi}, month = {4}, number = 10, pages = {3329-3339}, timestamp = {2023-06-15T22:49:36.000+0200}, title = {Detection of viral antibodies in camel sera using magnetic particle spectroscopy}, volume = 107, year = 2023 }