%0 Journal Article %1 endres2022development %A Endres, Leo M. %A Jungblut, Marvin %A Divyapicigil, Mustafa %A Sauer, Markus %A Stigloher, Christian %A Christodoulides, Myron %A Kim, Brandon J. %A Schubert-Unkmeir, Alexandra %D 2022 %J Fluids and Barriers of the CNS %K extern %N 1 %P 81-- %R 10.1186/s12987-022-00379-z %T Development of a multicellular in vitro model of the meningeal blood-CSF barrier to study Neisseria meningitidis infection %U https://doi.org/10.1186/s12987-022-00379-z %V 19 %X Bacterial meningitis is a life-threatening disease that occurs when pathogens such as Neisseria meningitidis cross the meningeal blood cerebrospinal fluid barrier (mBCSFB) and infect the meninges. Due to the human-specific nature of N. meningitidis, previous research investigating this complex host–pathogen interaction has mostly been done in vitro using immortalized brain endothelial cells (BECs) alone, which often do not retain relevant barrier properties in culture. Here, we developed physiologically relevant mBCSFB models using BECs in co-culture with leptomeningeal cells (LMCs) to examine N. meningitidis interaction.

@article{endres2022development, abstract = {Bacterial meningitis is a life-threatening disease that occurs when pathogens such as Neisseria meningitidis cross the meningeal blood cerebrospinal fluid barrier (mBCSFB) and infect the meninges. Due to the human-specific nature of N. meningitidis, previous research investigating this complex host–pathogen interaction has mostly been done in vitro using immortalized brain endothelial cells (BECs) alone, which often do not retain relevant barrier properties in culture. Here, we developed physiologically relevant mBCSFB models using BECs in co-culture with leptomeningeal cells (LMCs) to examine N. meningitidis interaction.}, added-at = {2022-11-14T09:03:34.000+0100}, author = {Endres, Leo M. and Jungblut, Marvin and Divyapicigil, Mustafa and Sauer, Markus and Stigloher, Christian and Christodoulides, Myron and Kim, Brandon J. and Schubert-Unkmeir, Alexandra}, biburl = {https://www.bibsonomy.org/bibtex/270d6df657194a51248e9947b2fe6cd7b/zam}, doi = {10.1186/s12987-022-00379-z}, interhash = {fafde0047e6dfb1320fe0ce17e936967}, intrahash = {70d6df657194a51248e9947b2fe6cd7b}, issn = {20458118}, journal = {Fluids and Barriers of the CNS}, keywords = {extern}, number = 1, pages = {81--}, refid = {Endres2022}, timestamp = {2022-11-14T09:04:26.000+0100}, title = {Development of a multicellular in vitro model of the meningeal blood-CSF barrier to study Neisseria meningitidis infection}, url = {https://doi.org/10.1186/s12987-022-00379-z}, volume = 19, year = 2022 }