%0 Journal Article %1 Morel2012 %A Morel, Mathieu %A Galas, Jean-Christophe %A Dahan, Maxime %A Studer, Vincent %D 2012 %I The Royal Society of Chemistry %J Lab Chip %K gradient microfluidics %N 7 %P 1340-1346 %R 10.1039/C2LC20994B %T Concentration landscape generators for shear free dynamic chemical stimulation %U http://dx.doi.org/10.1039/C2LC20994B %V 12 %X In this paper we first introduce a novel fabrication process, which allows for easy integration of thin track-etched nanoporous membranes, within 2D or 3D microchannel networks. In these networks, soluble chemical compounds can diffuse out of the channels through well-defined and spatially organized microfabricated porous openings. Interestingly, multiple micron-scale porous areas can be integrated in the same device and each of these areas can be connected to a different microfluidic channel and reservoir. We then present and characterize several membrane-based microdevices and their use for the generation of stable diffusible concentration gradients and complex dynamic chemical landscapes under shear free conditions. We also demonstrate how a simple flow-focusing geometry can be used to generate ön-demand" concentration profiles. In turn, these devices should provide an ideal experimental framework for high throughput cell-based assays: long term high-resolution video microscopy experiments can be performed, under multiple spatially and temporally controlled chemical conditions, with simple protocols and in a cell-friendly environment.

@article{Morel2012, abstract = {In this paper we first introduce a novel fabrication process{,} which allows for easy integration of thin track-etched nanoporous membranes{,} within 2D or 3D microchannel networks. In these networks{,} soluble chemical compounds can diffuse out of the channels through well-defined and spatially organized microfabricated porous openings. Interestingly{,} multiple micron-scale porous areas can be integrated in the same device and each of these areas can be connected to a different microfluidic channel and reservoir. We then present and characterize several membrane-based microdevices and their use for the generation of stable diffusible concentration gradients and complex dynamic chemical landscapes under shear free conditions. We also demonstrate how a simple flow-focusing geometry can be used to generate {"}on-demand{"} concentration profiles. In turn{,} these devices should provide an ideal experimental framework for high throughput cell-based assays: long term high-resolution video microscopy experiments can be performed{,} under multiple spatially and temporally controlled chemical conditions{,} with simple protocols and in a cell-friendly environment.}, added-at = {2013-01-08T16:23:25.000+0100}, author = {Morel, Mathieu and Galas, Jean-Christophe and Dahan, Maxime and Studer, Vincent}, biburl = {https://www.bibsonomy.org/bibtex/2309489cf20e183fc76a393ddbd412e3e/quantentunnel}, doi = {10.1039/C2LC20994B}, file = {Morel2012.pdf:Artikel/Morel2012.pdf:PDF}, groups = {public}, interhash = {fb1ba7fd685623374430c492c6b8b69c}, intrahash = {309489cf20e183fc76a393ddbd412e3e}, journal = {Lab Chip}, keywords = {gradient microfluidics}, number = 7, pages = {1340-1346}, publisher = {The Royal Society of Chemistry}, timestamp = {2013-04-16T10:24:22.000+0200}, title = {Concentration landscape generators for shear free dynamic chemical stimulation}, url = {http://dx.doi.org/10.1039/C2LC20994B}, username = {quantentunnel}, volume = 12, year = 2012 }