%0 Journal Article %1 citeulike:570602 %A Ballantyne, F. C. %A Williamson, J. %A Shapiro, D. %A Caslake, M. J. %A Perry, B. %D 1978 %J Clin Chem %K immunonephelometry apob %N 5 %P 788--792 %T Estimation of apolipoprotein B in man by immunonephelometry. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=647913 %V 24 %X We assessed a manual nephelometer incorporating a laser as light source and an optical system that detects forward scatter for use in estimating apolipoprotein B (apoB) by immunonephelometry. Comparisons were made with a less-sensitive, automated immunoprecipitation system and with a radioimmunoassay. ApoB, cholesterol, and triglycerides were estimated on plasma from 87 normal and hyperlipidemic subjects and on lipoprotein fractions from 28 of these plasmas. Regression analysis showed linear relationships between estimates of apoB concentration obtained by the three methods, but the mean concentrations were significantly different (pair difference t-test, P < 0.00 1). The manual nephelometer gave the highest, the radioimmunoassay the lowest results. The immunoprecipitation system gave excessively high sample blanks for most very-low-density lipoprotein fractions and for plasmas with high triglyceride concentrations. Results for apoB in plasma and low-density lipoprotein fractions correlated with cholesterol (P < 0.00 1) but with triglyceride concentrations.

@article{citeulike:570602, abstract = {We assessed a manual nephelometer incorporating a laser as light source and an optical system that detects forward scatter for use in estimating apolipoprotein B (apoB) by immunonephelometry. Comparisons were made with a less-sensitive, automated immunoprecipitation system and with a radioimmunoassay. ApoB, cholesterol, and triglycerides were estimated on plasma from 87 normal and hyperlipidemic subjects and on lipoprotein fractions from 28 of these plasmas. Regression analysis showed linear relationships between estimates of apoB concentration obtained by the three methods, but the mean concentrations were significantly different (pair difference t-test, P < 0.00 1). The manual nephelometer gave the highest, the radioimmunoassay the lowest results. The immunoprecipitation system gave excessively high sample blanks for most very-low-density lipoprotein fractions and for plasmas with high triglyceride concentrations. Results for apoB in plasma and low-density lipoprotein fractions correlated with cholesterol (P < 0.00 1) but with triglyceride concentrations.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Ballantyne, F. C. and Williamson, J. and Shapiro, D. and Caslake, M. J. and Perry, B.}, biburl = {https://www.bibsonomy.org/bibtex/20fce0be63e27398c41950d334e721401/biblio24}, citeulike-article-id = {570602}, interhash = {0260340f761c5d3f8ca95aacfa37b91d}, intrahash = {0fce0be63e27398c41950d334e721401}, issn = {0009-9147}, journal = {Clin Chem}, keywords = {immunonephelometry apob}, month = May, number = 5, pages = {788--792}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Estimation of apolipoprotein B in man by immunonephelometry.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=647913}, volume = 24, year = 1978 }