%0 Journal Article %1 Dischinger_2021 %A Dischinger, Ulrich %A Heckel, Tobias %A Bischler, Thorsten %A Hasinger, Julia %A Königsrainer, Malina %A Schmitt-Böhrer, Angelika %A Otto, Christoph %A Fassnacht, Martin %A Seyfried, Florian %A Hankir, Mohammed Khair %D 2021 %I MDPI AG %J Nutrients %K myown %N 1 %P 116 %R 10.3390/nu14010116 %T Roux-en-Y Gastric Bypass and Caloric Restriction but Not Gut Hormone-Based Treatments Profoundly Impact the Hypothalamic Transcriptome in Obese Rats %U https://doi.org/10.3390%2Fnu14010116 %V 14 %X Background: The hypothalamus is an important brain region for the regulation of energy balance. Roux-en-Y gastric bypass (RYGB) surgery and gut hormone-based treatments are known to reduce body weight, but their effects on hypothalamic gene expression and signaling pathways are poorly studied. Methods: Diet-induced obese male Wistar rats were randomized into the following groups: RYGB, sham operation, sham + body weight-matched (BWM) to the RYGB group, osmotic minipump delivering PYY3-36 (0.1 mg/kg/day), liraglutide s.c. (0.4 mg/kg/day), PYY3-36 + liraglutide, and saline. All groups (except BWM) were kept on a free choice of high- and low-fat diets. Four weeks after interventions, hypothalami were collected for RNA sequencing. Results: While rats in the RYGB, BWM, and PYY3-36 + liraglutide groups had comparable reductions in body weight, only RYGB and BWM treatment had a major impact on hypothalamic gene expression. In these groups, hypothalamic leptin receptor expression as well as the JAK-STAT, PI3K-Akt, and AMPK signaling pathways were upregulated. No significant changes could be detected in PYY3-36 + liraglutide-, liraglutide-, and PYY-treated groups. Conclusions: Despite causing similar body weight changes compared to RYGB and BWM, PYY3-36 + liraglutide treatment does not impact hypothalamic gene expression. Whether this striking difference is favorable or unfavorable to metabolic health in the long term requires further investigation. Keywords: PYY3-36; Roux-en-Y gastric bypass surgery; hypothalamic gene expression; liraglutide; obesity.

@article{Dischinger_2021, abstract = { Background: The hypothalamus is an important brain region for the regulation of energy balance. Roux-en-Y gastric bypass (RYGB) surgery and gut hormone-based treatments are known to reduce body weight, but their effects on hypothalamic gene expression and signaling pathways are poorly studied. Methods: Diet-induced obese male Wistar rats were randomized into the following groups: RYGB, sham operation, sham + body weight-matched (BWM) to the RYGB group, osmotic minipump delivering PYY3-36 (0.1 mg/kg/day), liraglutide s.c. (0.4 mg/kg/day), PYY3-36 + liraglutide, and saline. All groups (except BWM) were kept on a free choice of high- and low-fat diets. Four weeks after interventions, hypothalami were collected for RNA sequencing. Results: While rats in the RYGB, BWM, and PYY3-36 + liraglutide groups had comparable reductions in body weight, only RYGB and BWM treatment had a major impact on hypothalamic gene expression. In these groups, hypothalamic leptin receptor expression as well as the JAK-STAT, PI3K-Akt, and AMPK signaling pathways were upregulated. No significant changes could be detected in PYY3-36 + liraglutide-, liraglutide-, and PYY-treated groups. Conclusions: Despite causing similar body weight changes compared to RYGB and BWM, PYY3-36 + liraglutide treatment does not impact hypothalamic gene expression. Whether this striking difference is favorable or unfavorable to metabolic health in the long term requires further investigation. Keywords: PYY3-36; Roux-en-Y gastric bypass surgery; hypothalamic gene expression; liraglutide; obesity. }, added-at = {2022-01-13T08:43:33.000+0100}, author = {Dischinger, Ulrich and Heckel, Tobias and Bischler, Thorsten and Hasinger, Julia and Königsrainer, Malina and Schmitt-Böhrer, Angelika and Otto, Christoph and Fassnacht, Martin and Seyfried, Florian and Hankir, Mohammed Khair}, biburl = {https://www.bibsonomy.org/bibtex/2ccc9b6f47308f8f514e2e11a1c86a037/cusysmed}, day = 28, doi = {10.3390/nu14010116}, interhash = {05ccf6199c9108d333ff1bd30464cc22}, intrahash = {ccc9b6f47308f8f514e2e11a1c86a037}, journal = {Nutrients}, keywords = {myown}, month = dec, number = 1, pages = 116, publisher = {MDPI AG}, timestamp = {2024-04-17T09:46:24.000+0200}, title = {Roux-en-Y Gastric Bypass and Caloric Restriction but Not Gut Hormone-Based Treatments Profoundly Impact the Hypothalamic Transcriptome in Obese Rats}, url = {https://doi.org/10.3390%2Fnu14010116}, volume = 14, year = 2021 }