%0 Journal Article %1 li2014mir1505p %A Li, Tao %A Xie, Junjie %A Shen, Chuan %A Cheng, Dongfeng %A Shi, Yuan %A Wu, Zhichong %A Zhan, Qian %A Deng, Xiaxing %A Chen, Hao %A Shen, Baiyong %A Peng, Chenghong %A Li, Hongwei %A Zhu, Zhecheng %D 2014 %J PLOS One %K 3 background cancer epigenetics mirnas %N 12 %P 3115577 %R 10.1371/journal.pone.0115577 %T miR-150-5p Inhibits Hepatoma Cell Migration and Invasion by Targeting MMP14 %U http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0115577 %V 9 %X Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. Despite progress in diagnostics and treatment of HCC, its prognosis remains poor because the molecular mechanisms underlying hepatocarcinogenesis are not well understood. In the study, we focused on identifying the role of miRNAs in HCC progression. miRNA microarray was used to analyze the differentially expressed miRNAs, and the results were validated by qPCR. We found that the miR-150-5p expression is down-regulated in HCC tissues compared with pair non-tumor tissues. miR-150-5p expression is also decreased in metastatic cancer tissues compared with pair primary tissues, indicating that miR-150-5p may be involved in HCC metastasis. Functionally, miR-150-5p inhibition significantly promotes hepatoma cell migration and invasion, whereas miR-150-5p overexpression suppresses cancer cell migration and invasion in vitro. The matrix metalloproteinase 14 (MMP14) is identified as a new target gene of miR-150-5p. miR-150-5p markedly inhibits MMP14 expression in hepatoma cells, and miR-150-5p expression is negative correlation with MMP14 expression in vivo. More important, re-expression of MMP14 in hepatoma cells partially reverses the effect of miR-150-5p in inhibiting cell invasion.

@article{li2014mir1505p, abstract = {Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. Despite progress in diagnostics and treatment of HCC, its prognosis remains poor because the molecular mechanisms underlying hepatocarcinogenesis are not well understood. In the study, we focused on identifying the role of miRNAs in HCC progression. miRNA microarray was used to analyze the differentially expressed miRNAs, and the results were validated by qPCR. We found that the miR-150-5p expression is down-regulated in HCC tissues compared with pair non-tumor tissues. miR-150-5p expression is also decreased in metastatic cancer tissues compared with pair primary tissues, indicating that miR-150-5p may be involved in HCC metastasis. Functionally, miR-150-5p inhibition significantly promotes hepatoma cell migration and invasion, whereas miR-150-5p overexpression suppresses cancer cell migration and invasion in vitro. The matrix metalloproteinase 14 (MMP14) is identified as a new target gene of miR-150-5p. miR-150-5p markedly inhibits MMP14 expression in hepatoma cells, and miR-150-5p expression is negative correlation with MMP14 expression in vivo. More important, re-expression of MMP14 in hepatoma cells partially reverses the effect of miR-150-5p in inhibiting cell invasion.}, added-at = {2017-10-04T03:54:18.000+0200}, author = {Li, Tao and Xie, Junjie and Shen, Chuan and Cheng, Dongfeng and Shi, Yuan and Wu, Zhichong and Zhan, Qian and Deng, Xiaxing and Chen, Hao and Shen, Baiyong and Peng, Chenghong and Li, Hongwei and Zhu, Zhecheng}, biburl = {https://www.bibsonomy.org/bibtex/24838e2d479bdff4282a8a778d2a4710f/artheibault}, description = {This article provides useful information about the function of miR-150-5p. This microRNA was one the article by Shi et al. found significant in hepatocellular carcinoma. This article provided information on what it actually did.}, doi = {10.1371/journal.pone.0115577}, interhash = {0679c53e87dad0b01ed14951013d0c68}, intrahash = {4838e2d479bdff4282a8a778d2a4710f}, journal = {PLOS One}, keywords = {3 background cancer epigenetics mirnas}, number = 12, pages = 3115577, timestamp = {2017-10-25T22:17:32.000+0200}, title = {miR-150-5p Inhibits Hepatoma Cell Migration and Invasion by Targeting MMP14}, url = {http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0115577}, volume = 9, year = 2014 }