%0 Journal Article %1 RN62 %A Ravanal, M.C. %A Espinosa, Y. %A Rosa, L. %A Vaca, I. %A Polanco, R. %A Eyzaguirre, J. %A Levican, G. %A Chavez, R. %D 2012 %J Mycoscience %K cloning dqcauchile expression, genes, glucose heterologous induction, penicillium purpurogenum, secretion, transformation, trichoderma-reesei, xylanase, %N 2 %P 152-155 %R 10.1007/s10267-011-0144-1 %T Glucose-Induced Production of a Penicillium Purpurogenum Xylanase by Aspergillus Nidulans %U /brokenurl#<Go to ISI>://WOS:000301638800010 %V 53 %X The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0.72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.

@article{RN62, abstract = {The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0.72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.}, added-at = {2019-12-04T03:57:35.000+0100}, author = {Ravanal, M.C. and Espinosa, Y. and Rosa, L. and Vaca, I. and Polanco, R. and Eyzaguirre, J. and Levican, G. and Chavez, R.}, biburl = {https://www.bibsonomy.org/bibtex/21c9e182bbb8104a15e14baf3637f5a1a/dqcauchile}, doi = {10.1007/s10267-011-0144-1}, interhash = {073e172b9af0a5ece4299384ffcc462d}, intrahash = {1c9e182bbb8104a15e14baf3637f5a1a}, issn = {1340-3540}, journal = {Mycoscience}, keywords = {cloning dqcauchile expression, genes, glucose heterologous induction, penicillium purpurogenum, secretion, transformation, trichoderma-reesei, xylanase,}, number = 2, pages = {152-155}, timestamp = {2019-12-04T03:58:17.000+0100}, title = {Glucose-Induced Production of a Penicillium Purpurogenum Xylanase by Aspergillus Nidulans}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000301638800010}, volume = 53, year = 2012 }