%0 Journal Article %1 Myers:2000aa %A Myers, E W %A Sutton, G G %A Delcher, A L %A Dew, I M %A Fasulo, D P %A Flanigan, M J %A Kravitz, S A %A Mobarry, C M %A Reinert, K H %A Remington, K A %A Anson, E L %A Bolanos, R A %A Chou, H H %A Jordan, C M %A Halpern, A L %A Lonardi, S %A Beasley, E M %A Brandon, R C %A Chen, L %A Dunn, P J %A Lai, Z %A Liang, Y %A Nusskern, D R %A Zhan, M %A Zhang, Q %A Zheng, X %A Rubin, G M %A Adams, M D %A Venter, J C %D 2000 %J Science %K Acid, Algorithms, Analysis, Animals, Biology Chromatin, Chromosome Computational Contig DNA, Data, Drosophila Euchromatin, Genes, Genome, Heterochromatin, Insect, Mapping, Molecular Nucleic Physical Repetitive Sequence Sequences, Sites, Tagged melanogaster, %N 5461 %P 2196--204 %T A whole-genome assembly of Drosophila %V 287 %X We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9- megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99. 99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community.

@article{Myers:2000aa, abstract = {We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9- megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99. 99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community.}, added-at = {2007-09-17T20:19:41.000+0200}, affiliation = {Celera Genomics, Inc., 45 West Gude Drive, Rockville, MD 20850, USA. Gene.Myers@celera.com}, author = {Myers, E W and Sutton, G G and Delcher, A L and Dew, I M and Fasulo, D P and Flanigan, M J and Kravitz, S A and Mobarry, C M and Reinert, K H and Remington, K A and Anson, E L and Bolanos, R A and Chou, H H and Jordan, C M and Halpern, A L and Lonardi, S and Beasley, E M and Brandon, R C and Chen, L and Dunn, P J and Lai, Z and Liang, Y and Nusskern, D R and Zhan, M and Zhang, Q and Zheng, X and Rubin, G M and Adams, M D and Venter, J C}, biburl = {https://www.bibsonomy.org/bibtex/211f0d08db68b4f73ce33ffab95ffef98/dzerbino}, interhash = {0cd5e9ee59dbf182e8e9476fa03c93e0}, intrahash = {11f0d08db68b4f73ce33ffab95ffef98}, issue = {5461}, journal = {Science}, keywords = {Acid, Algorithms, Analysis, Animals, Biology Chromatin, Chromosome Computational Contig DNA, Data, Drosophila Euchromatin, Genes, Genome, Heterochromatin, Insect, Mapping, Molecular Nucleic Physical Repetitive Sequence Sequences, Sites, Tagged melanogaster,}, language = {English}, local-url = {file://localhost/Users/danielzerbino/Documents/Papers/2000/Myers/Science%202000%20Myers.pdf}, month = Mar, number = 5461, pages = {2196--204}, pii = {8395}, pmid = {10731133}, timestamp = {2007-09-17T20:19:42.000+0200}, title = {A whole-genome assembly of Drosophila}, uri = {papers://055852FE-1648-42FE-91D0-8CA474D2B905/Paper/p20}, volume = 287, year = 2000 }