%0 Journal Article %1 C2LC21285D %A Han, Sewoon %A Yang, Kisuk %A Shin, Yoojin %A Lee, Jung Seung %A Kamm, Roger D. %A Chung, Seok %A Cho, Seung-Woo %D 2012 %I The Royal Society of Chemistry %J Lab Chip %K Britta differentiation microfluidics stemcell %N 13 %P 2305-2308 %R 10.1039/C2LC21285D %T Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device %U http://dx.doi.org/10.1039/C2LC21285D %V 12 %X Here, we report a unique method to quantify the effects of in vivo-like extracellular matrix (ECM) for guiding differentiation of neural stem cells (NSCs) in three-dimensional (3D) microenvironments using quantitative real-time polymerase chain reaction (qRT-PCR). We successfully monitored and quantified differentiation of NSCs in small volume ECMs and found that differentiation of NSCs, especially those differentiating towards neuronal and oligodendrocytic lineages, is significantly enhanced by 3D microenvironments reconstituted in the microfluidic channels.

@article{C2LC21285D, abstract = {Here{,} we report a unique method to quantify the effects of in vivo-like extracellular matrix (ECM) for guiding differentiation of neural stem cells (NSCs) in three-dimensional (3D) microenvironments using quantitative real-time polymerase chain reaction (qRT-PCR). We successfully monitored and quantified differentiation of NSCs in small volume ECMs and found that differentiation of NSCs{,} especially those differentiating towards neuronal and oligodendrocytic lineages{,} is significantly enhanced by 3D microenvironments reconstituted in the microfluidic channels.}, added-at = {2012-09-25T16:13:50.000+0200}, author = {Han, Sewoon and Yang, Kisuk and Shin, Yoojin and Lee, Jung Seung and Kamm, Roger D. and Chung, Seok and Cho, Seung-Woo}, biburl = {https://www.bibsonomy.org/bibtex/266faf8ef4fd1344c4ca1913e3ac5ebfc/ak15}, description = {Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device - Lab on a Chip (RSC Publishing)}, doi = {10.1039/C2LC21285D}, interhash = {0e3667ca6e2f7add94cb1861671dbed4}, intrahash = {66faf8ef4fd1344c4ca1913e3ac5ebfc}, journal = {Lab Chip}, keywords = {Britta differentiation microfluidics stemcell}, number = 13, pages = {2305-2308}, publisher = {The Royal Society of Chemistry}, timestamp = {2012-09-25T16:13:50.000+0200}, title = {Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device}, url = {http://dx.doi.org/10.1039/C2LC21285D}, volume = 12, year = 2012 }