Abstract
The recent cloning of the complementary DNAs and/or genes for several
receptors linked to guanine nucleotide regulatory proteins including
the adrenergic receptors (alpha 1, alpha 2A, alpha 2B, beta 1, beta
2), several subtypes of the muscarinic cholinergic receptors, and
the visual 'receptor' rhodopsin has revealed considerable similarity
in the primary structure of these proteins. In addition, all of these
proteins contain seven putative transmembrane alpha-helices. We have
previously described a genomic clone, G-21, isolated by cross-hybridization
at reduced stringency with a full length beta 2-adrenergic receptor
probe. This clone contains an intronless gene which, because of its
striking sequence resemblance to the adrenergic receptors, is presumed
to encode a G-protein-coupled receptor. Previous attempts to identify
this putative receptor by expression studies have failed. We now
report that the protein product of the genomic clone, G21, transiently
expressed in monkey kidney cells has all the typical ligand-binding
characteristics of the 5-hydroxytryptamine (5-HT1A) receptor.
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