%0 Journal Article %1 ahmed18october2018isolation %A Ahmed, Madiha %A Kondratyuk, Tamara P. %A Pezzuto, John M. %A Groundwater, Paul W. %A ul Haq, Ihsan %D 18October, 2018 %J Journal of Oncology Research and Therapy %K Dilatata L Penta-Cyclic Quercus Triterpenoids Two %N 06 %P 06 %T Isolation of Two Penta-Cyclic Triterpenoids from Quercus DilatataL. and Evaluation of Their Chemo-Preventive Potential %U https://www.gavinpublishers.com/articles/Research-Article/Journal-of-Oncology-Research-and-Therapy-ISSN-2574-710X/isolation-of-two-penta-cyclic-triterpenoids-from-quercus-dilatataL-and-evaluation-of-their-chemo-preventive-potential %V 2018 %X Natural products especially medicinal plants have served as reserve of bioactive secondary metabolites which are in continuous phase of isolation and identification for therapeutic purpose. Present study was designed to explore the phytochemical riches of Quercus dilatataL. Two pentacyclic triterpenoids named as friedelin and epifriedelinol were isolated from n-hexane fraction of crude methanolic extract of Quercus dilatataaerial parts by bioassay guided separation techniques. Normal phase silica gel column chromatography was used for the separation of compounds. The structures were mainly characterized by means of nuclear magnetic resonance and electron ionization mass spectrometry, after comparing with the data from literature. These compounds have been isolated for the first time from Q. dilatata. Among the two triterpenoids, friedelin showed substantial inhibition of nitric oxide production in lipopolysaccharide-activated murine macrophage raw 264.7 cells (63.0 ± 5.3%). It also significantly inhibited tumor necrosis factor-α activated nuclear factor-κB in stable transfected human embryonic kidney cells 293 (65.2 ± 3.2%). Survival rates of cells in both assays were 92.3 ± 2.3% and 100 ± 8.4% respectively. Further it also showed moderate protein kinase inhibitory activity against mutated Streptomyces 85E strain (7.0 ± 0.5 bald zone of inhibition) in hyphae formation inhibition assay. These results suggest the possible applications of friedelin as a good chemo-preventive agent.

@article{ahmed18october2018isolation, abstract = {Natural products especially medicinal plants have served as reserve of bioactive secondary metabolites which are in continuous phase of isolation and identification for therapeutic purpose. Present study was designed to explore the phytochemical riches of Quercus dilatataL. Two pentacyclic triterpenoids named as friedelin and epifriedelinol were isolated from n-hexane fraction of crude methanolic extract of Quercus dilatataaerial parts by bioassay guided separation techniques. Normal phase silica gel column chromatography was used for the separation of compounds. The structures were mainly characterized by means of nuclear magnetic resonance and electron ionization mass spectrometry, after comparing with the data from literature. These compounds have been isolated for the first time from Q. dilatata. Among the two triterpenoids, friedelin showed substantial inhibition of nitric oxide production in lipopolysaccharide-activated murine macrophage raw 264.7 cells (63.0 ± 5.3%). It also significantly inhibited tumor necrosis factor-α activated nuclear factor-κB in stable transfected human embryonic kidney cells 293 (65.2 ± 3.2%). Survival rates of cells in both assays were 92.3 ± 2.3% and 100 ± 8.4% respectively. Further it also showed moderate protein kinase inhibitory activity against mutated Streptomyces 85E strain (7.0 ± 0.5 bald zone of inhibition) in hyphae formation inhibition assay. These results suggest the possible applications of friedelin as a good chemo-preventive agent.}, added-at = {2019-07-06T08:55:06.000+0200}, author = {Ahmed, Madiha and Kondratyuk, Tamara P. and Pezzuto, John M. and Groundwater, Paul W. and ul Haq, Ihsan}, biburl = {https://www.bibsonomy.org/bibtex/23e5c1b8081866c258a1fc9a37bab0e7a/alyssacarter}, interhash = {1c3e370fade0be7903a6c6ae566bc969}, intrahash = {3e5c1b8081866c258a1fc9a37bab0e7a}, issn = {ISSN:2574-710X}, journal = {Journal of Oncology Research and Therapy}, keywords = {Dilatata L Penta-Cyclic Quercus Triterpenoids Two}, language = {English}, month = {18October}, number = 06, pages = 06, timestamp = {2019-07-06T08:55:06.000+0200}, title = {Isolation of Two Penta-Cyclic Triterpenoids from Quercus DilatataL. and Evaluation of Their Chemo-Preventive Potential}, url = {https://www.gavinpublishers.com/articles/Research-Article/Journal-of-Oncology-Research-and-Therapy-ISSN-2574-710X/isolation-of-two-penta-cyclic-triterpenoids-from-quercus-dilatataL-and-evaluation-of-their-chemo-preventive-potential}, volume = 2018, year = {18October, 2018} }