Abstract
A high-capacity system was developed to monitor the expression of
many genes in parallel. Microarrays prepared by high-speed robotic
printing of complementary DNAs on glass were used for quantitative
expression measurements of the corresponding genes. Because of the
small format and high density of the arrays, hybridization volumes
of 2 microliters could be used that enabled detection of rare transcripts
in probe mixtures derived from 2 micrograms of total cellular messenger
RNA. Differential expression measurements of 45 Arabidopsis genes
were made by means of simultaneous, two-color fluorescence hybridization.
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