%0 Journal Article %1 cotugno2021virological %A Cotugno, Nicola %A Ruggiero, Alessandra %A Bonfante, Francesco %A Petrara, Maria Raffaella %A Zicari, Sonia %A Pascucci, Giuseppe Rubens %A Zangari, Paola %A De Ioris, Maria Antonietta %A Santilli, Veronica %A Manno, E.C %A Amodio, Donato %A Bortolami, Alessio %A Pagliari, Matteo %A Concato, Carlo %A Linardos, Giulia %A Campana, Andrea %A Donà, Daniele %A Giaquinto, Carlo %A Bernardi, Stefania %A Romani, Lorenza %A Pansa, Paola %A Chiurchiú, Sara %A Finocchi, Andrea %A Cancrini, Caterina %A Lancella, Laura %A Cursi, Laura %A De Luca, Maia %A Cutrera, Renato %A Sessa, Libera %A Morrocchi, Elena %A Medri, Chiara %A Putignani, Lorenza %A Calò Carducci, F.I %A D’Argenio, Patrizia %A Ciofi degli Atti, Marta %A D’Amore, Carmen %A Piccioni, Livia %A Di Giuseppe, Martina %A Jenkner, Alessandro %A Giancotta, Carmela %A Krzysztofiak, Andrzej %A Brodin, Petter %A Rossi, Paolo %A De Rossi, Anita %A Palma, Paolo %B Cell Reports %D 2021 %I Elsevier %J Cell Reports %K SARS-Cov-2 %N 11 %R 10.1016/j.celrep.2021.108852 %T Virological and immunological features of SARS-CoV-2-infected children who develop neutralizing antibodies %U https://doi.org/10.1016/j.celrep.2021.108852 %V 34 %X As the global COVID-19 pandemic progresses, it is paramount to gain knowledge on adaptive immunity to SARS-CoV-2 in children to define immune correlates of protection upon immunization or infection. We analyzed anti-SARS-CoV-2 antibodies and their neutralizing activity (PRNT) in 66 COVID-19-infected children at 7 (±2) days after symptom onset. Individuals with specific humoral responses presented faster virus clearance and lower viral load associated with a reduced in vitro infectivity. We demonstrated that the frequencies of SARS-CoV-2-specific CD4+CD40L+ T cells and Spike-specific B cells were associated with the anti-SARS-CoV-2 antibodies and the magnitude of neutralizing activity. The plasma proteome confirmed the association between cellular and humoral SARS-CoV-2 immunity, and PRNT+ patients show higher viral signal transduction molecules (SLAMF1, CD244, CLEC4G). This work sheds lights on cellular and humoral anti-SARS-CoV-2 responses in children, which may drive future vaccination trial endpoints and quarantine measures policies.

@article{cotugno2021virological, abstract = {As the global COVID-19 pandemic progresses, it is paramount to gain knowledge on adaptive immunity to SARS-CoV-2 in children to define immune correlates of protection upon immunization or infection. We analyzed anti-SARS-CoV-2 antibodies and their neutralizing activity (PRNT) in 66 COVID-19-infected children at 7 (±2) days after symptom onset. Individuals with specific humoral responses presented faster virus clearance and lower viral load associated with a reduced in vitro infectivity. We demonstrated that the frequencies of SARS-CoV-2-specific CD4+CD40L+ T cells and Spike-specific B cells were associated with the anti-SARS-CoV-2 antibodies and the magnitude of neutralizing activity. The plasma proteome confirmed the association between cellular and humoral SARS-CoV-2 immunity, and PRNT+ patients show higher viral signal transduction molecules (SLAMF1, CD244, CLEC4G). This work sheds lights on cellular and humoral anti-SARS-CoV-2 responses in children, which may drive future vaccination trial endpoints and quarantine measures policies.}, added-at = {2021-03-17T08:33:32.000+0100}, author = {Cotugno, Nicola and Ruggiero, Alessandra and Bonfante, Francesco and Petrara, Maria Raffaella and Zicari, Sonia and Pascucci, Giuseppe Rubens and Zangari, Paola and De Ioris, Maria Antonietta and Santilli, Veronica and Manno, E.C and Amodio, Donato and Bortolami, Alessio and Pagliari, Matteo and Concato, Carlo and Linardos, Giulia and Campana, Andrea and Donà, Daniele and Giaquinto, Carlo and Bernardi, Stefania and Romani, Lorenza and Pansa, Paola and Chiurchiú, Sara and Finocchi, Andrea and Cancrini, Caterina and Lancella, Laura and Cursi, Laura and De Luca, Maia and Cutrera, Renato and Sessa, Libera and Morrocchi, Elena and Medri, Chiara and Putignani, Lorenza and Calò Carducci, F.I and D’Argenio, Patrizia and Ciofi degli Atti, Marta and D’Amore, Carmen and Piccioni, Livia and Di Giuseppe, Martina and Jenkner, Alessandro and Giancotta, Carmela and Krzysztofiak, Andrzej and Brodin, Petter and Rossi, Paolo and De Rossi, Anita and Palma, Paolo}, biburl = {https://www.bibsonomy.org/bibtex/2a14d408ba68ac9114c3d3793ca3809b2/lkanth}, booktitle = {Cell Reports}, comment = {doi: 10.1016/j.celrep.2021.108852}, description = {Virological and immunological features of SARS-CoV-2-infected children who develop neutralizing antibodies: Cell Reports}, doi = {10.1016/j.celrep.2021.108852}, interhash = {28c1f308c56a2bbfad1fed10b235aa23}, intrahash = {a14d408ba68ac9114c3d3793ca3809b2}, issn = {22111247}, journal = {Cell Reports}, keywords = {SARS-Cov-2}, month = mar, number = 11, publisher = {Elsevier}, timestamp = {2021-03-17T08:33:32.000+0100}, title = {Virological and immunological features of SARS-CoV-2-infected children who develop neutralizing antibodies}, url = {https://doi.org/10.1016/j.celrep.2021.108852}, volume = 34, year = 2021 }