%0 Journal Article %1 borrow_non-culture_1997 %A Borrow, R %A Claus, H %A Guiver, M %A Smart, L %A Jones, D M %A Kaczmarski, E B %A Frosch, M %A Fox, A J %D 1997 %J Epidemiology and Infection %K , Assay, Bacterial, Base Chain Data, Fragment Humans, Immunosorbent Infections, Length, Meningococcal Molecular Neisseria Polymerase Polymorphism, Reaction, Reproducibility Restriction Results, Sensitivity Sequence Sequence, Serotyping, Sialyltransferases Specificity, and bacterial_capsules meningitidis, of {DNA}, {Enzyme-Linked} %N 2 %P 111--117 %T Non-culture diagnosis and serogroup determination of meningococcal B and C infection by a sialyltransferase (siaD) PCR ELISA %U http://www.ncbi.nlm.nih.gov/pubmed/9129587 %V 118 %X Rapid, non-culture, serogroup determination of meningococcal infection is important in contact management where vaccination may be possible. The impending availability of polysaccharide-protein conjugate vaccines for serogroup C disease requires maximal case ascertainment, with serogroup determination, at a time when the number of culture confirmed meningococcal infections is decreasing. A polymerase chain reaction assay (PCR), based on a restriction fragment length polymorphism (RFLP) in the meningococcal serogroup B and C sialytransferase (siaD) gene, was developed to combine the non-culture diagnosis of meningococcal infection from CSF, whole blood and serum with serogroup (B and C) identification. The PCR assay was adapted to an ELISA format incorporating hybridization with serogroup-specific B and C oligonucleotide probes. Specificity for CSFs was 100\% and sensitivities were respectively 81, 63 and 30\% for CSFs, whole blood and sera. The serogroup-specific PCR ELISA is a significant addition to currently available tests for non-culture diagnosis of meningococcal infection and outbreak investigation.

@article{borrow_non-culture_1997, abstract = {Rapid, non-culture, serogroup determination of meningococcal infection is important in contact management where vaccination may be possible. The impending availability of polysaccharide-protein conjugate vaccines for serogroup C disease requires maximal case ascertainment, with serogroup determination, at a time when the number of culture confirmed meningococcal infections is decreasing. A polymerase chain reaction assay {(PCR)}, based on a restriction fragment length polymorphism {(RFLP)} in the meningococcal serogroup B and C sialytransferase {(siaD)} gene, was developed to combine the non-culture diagnosis of meningococcal infection from {CSF}, whole blood and serum with serogroup {(B} and C) identification. The {PCR} assay was adapted to an {ELISA} format incorporating hybridization with serogroup-specific B and C oligonucleotide probes. Specificity for {CSFs} was 100\% and sensitivities were respectively 81, 63 and 30\% for {CSFs}, whole blood and sera. The serogroup-specific {PCR} {ELISA} is a significant addition to currently available tests for non-culture diagnosis of meningococcal infection and outbreak investigation.}, added-at = {2011-06-24T11:21:47.000+0200}, author = {Borrow, R and Claus, H and Guiver, M and Smart, L and Jones, D M and Kaczmarski, E B and Frosch, M and Fox, A J}, biburl = {https://www.bibsonomy.org/bibtex/2bd84ede9218c09c1434c24f8b602a135/ag_vogel}, interhash = {3207f8fbe4fde529cacc289045c32965}, intrahash = {bd84ede9218c09c1434c24f8b602a135}, issn = {0950-2688}, journal = {Epidemiology and Infection}, keywords = {, Assay, Bacterial, Base Chain Data, Fragment Humans, Immunosorbent Infections, Length, Meningococcal Molecular Neisseria Polymerase Polymorphism, Reaction, Reproducibility Restriction Results, Sensitivity Sequence Sequence, Serotyping, Sialyltransferases Specificity, and bacterial_capsules meningitidis, of {DNA}, {Enzyme-Linked}}, month = apr, note = {{PMID:} 9129587}, number = 2, pages = {111--117}, timestamp = {2011-08-08T15:45:43.000+0200}, title = {Non-culture diagnosis and serogroup determination of meningococcal B and C infection by a sialyltransferase {(siaD)} {PCR} {ELISA}}, url = {http://www.ncbi.nlm.nih.gov/pubmed/9129587}, volume = 118, year = 1997 }