%0 Journal Article %1 guzeldeger2015guard %A Guzel Deger, Aysin %A Scherzer, Sonke %A Nuhkat, Maris %A Kedzierska, Justyna %A Kollist, Hannes %A Brosche, Mikael %A Unyayar, Serpil %A Boudsocq, Marie %A Hedrich, Rainer %A Roelfsema, M. Rob G. %C Molecular Plant Physiology and Biophysics, Julius-von-Sachs Institute for Biosciences, Biocenter, University of Wurzburg, Julius-von-Sachs-Platz 2, D-97082, Wurzburg, Germany. %D 2015 %J The New phytologist %K MAMP S-type SLAC1 SLAH3 anion-channel flg22 guard-cell %N 1 %P 162-73 %R 10.1111/nph.13435 %T Guard cell SLAC1-type anion channels mediate flagellin-induced stomatal closure %U http://onlinelibrary.wiley.com/doi/10.1111/nph.13435/full %V 208 %X During infection plants recognize microbe-associated molecular patterns (MAMPs), and this leads to stomatal closure. This study analyzes the molecular mechanisms underlying this MAMP response and its interrelation with ABA signaling. Stomata in intact Arabidopsis thaliana plants were stimulated with the bacterial MAMP flg22, or the stress hormone ABA, by using the noninvasive nanoinfusion technique. Intracellular double-barreled microelectrodes were applied to measure the activity of plasma membrane ion channels. Flg22 induced rapid stomatal closure and stimulated the SLAC1 and SLAH3 anion channels in guard cells. Loss of both channels resulted in cells that lacked flg22-induced anion channel activity and stomata that did not close in response to flg22 or ABA. Rapid flg22-dependent stomatal closure was impaired in plants that were flagellin receptor (FLS2)-deficient, as well as in the ost1-2 (Open Stomata 1) mutant, which lacks a key ABA-signaling protein kinase. By contrast, stomata of the ABA protein phosphatase mutant abi1-1 (ABscisic acid Insensitive 1) remained flg22-responsive. These data suggest that the initial steps in flg22 and ABA signaling are different, but that the pathways merge at the level of OST1 and lead to activation of SLAC1 and SLAH3 anion channels.

@article{guzeldeger2015guard, abstract = { During infection plants recognize microbe-associated molecular patterns (MAMPs), and this leads to stomatal closure. This study analyzes the molecular mechanisms underlying this MAMP response and its interrelation with ABA signaling. Stomata in intact Arabidopsis thaliana plants were stimulated with the bacterial MAMP flg22, or the stress hormone ABA, by using the noninvasive nanoinfusion technique. Intracellular double-barreled microelectrodes were applied to measure the activity of plasma membrane ion channels. Flg22 induced rapid stomatal closure and stimulated the SLAC1 and SLAH3 anion channels in guard cells. Loss of both channels resulted in cells that lacked flg22-induced anion channel activity and stomata that did not close in response to flg22 or ABA. Rapid flg22-dependent stomatal closure was impaired in plants that were flagellin receptor (FLS2)-deficient, as well as in the ost1-2 (Open Stomata 1) mutant, which lacks a key ABA-signaling protein kinase. By contrast, stomata of the ABA protein phosphatase mutant abi1-1 (ABscisic acid Insensitive 1) remained flg22-responsive. These data suggest that the initial steps in flg22 and ABA signaling are different, but that the pathways merge at the level of OST1 and lead to activation of SLAC1 and SLAH3 anion channels.}, added-at = {2017-04-04T13:59:41.000+0200}, address = {Molecular Plant Physiology and Biophysics, Julius-von-Sachs Institute for Biosciences, Biocenter, University of Wurzburg, Julius-von-Sachs-Platz 2, D-97082, Wurzburg, Germany.}, author = {Guzel Deger, Aysin and Scherzer, Sonke and Nuhkat, Maris and Kedzierska, Justyna and Kollist, Hannes and Brosche, Mikael and Unyayar, Serpil and Boudsocq, Marie and Hedrich, Rainer and Roelfsema, M. Rob G.}, biburl = {https://www.bibsonomy.org/bibtex/2714f3496fd35d4050897ba8b0ce25dfe/robroelfsema}, doi = {10.1111/nph.13435}, interhash = {386175b36cbac2e47a42632cc16e9d8f}, intrahash = {714f3496fd35d4050897ba8b0ce25dfe}, issn = {14698137}, journal = {The New phytologist}, keywords = {MAMP S-type SLAC1 SLAH3 anion-channel flg22 guard-cell}, number = 1, pages = {162-73}, refid = {11}, timestamp = {2017-04-04T13:59:41.000+0200}, title = {Guard cell SLAC1-type anion channels mediate flagellin-induced stomatal closure}, url = {http://onlinelibrary.wiley.com/doi/10.1111/nph.13435/full}, volume = 208, year = 2015 }