%0 Journal Article %1 wangtumor %A Wang, Qianghu %A Hu, Baoli %A Hu, Xin %A Kim, Hoon %A Squatrito, Massimo %A Scarpace, Lisa %A deCarvalho, Ana C. %A Lyu, Sali %A Li, Pengping %A Li, Yan %A Barthel, Floris %A Cho, Hee Jin %A Lin, Yu-Hsi %A Satani, Nikunj %A Martinez-Ledesma, Emmanuel %A Zheng, Siyuan %A Chang, Edward %A Sauvé, Charles-Etienne Gabriel %A Olar, Adriana %A Lan, Zheng D. %A Finocchiaro, Gaetano %A Phillips, Joanna J. %A Berger, Mitchel S. %A Gabrusiewicz, Konrad R. %A Wang, Guocan %A Eskilsson, Eskil %A Hu, Jian %A Mikkelsen, Tom %A DePinho, Ronald A. %A Muller, Florian %A Heimberger, Amy B. %A Sulman, Erik P. %A Nam, Do-Hyun %A Verhaak, Roel G.W %B Cancer Cell %D 2017 %I Elsevier %J Cancer Cell %K PAYWALL cancer glioma microenvironment %N 1 %P 42--56.e6 %R 10.1016/j.ccell.2017.06.003 %T Tumor Evolution of Glioma-Intrinsic Gene Expression Subtypes Associates with Immunological Changes in the Microenvironment %U http://dx.doi.org/10.1016/j.ccell.2017.06.003 %V 32 %X We leveraged IDH wild-type glioblastomas, derivative neurospheres, and single-cell gene expression profiles to define three tumor-intrinsic transcriptional subtypes designated as proneural, mesenchymal, and classical. Transcriptomic subtype multiplicity correlated with increased intratumoral heterogeneity and presence of tumor microenvironment. In silico cell sorting identified macrophages/microglia, CD4+ T lymphocytes, and neutrophils in the glioma microenvironment. NF1 deficiency resulted in increased tumor-associated macrophages/microglia infiltration. Longitudinal transcriptome analysis showed that expression subtype is retained in 55% of cases. Gene signature-based tumor microenvironment inference revealed a decrease in invading monocytes and a subtype-dependent increase in macrophages/microglia cells upon disease recurrence. Hypermutation at diagnosis or at recurrence associated with CD8+ T cell enrichment. Frequency of M2 macrophages detection associated with short-term relapse after radiation therapy.

@article{wangtumor, abstract = {We leveraged IDH wild-type glioblastomas, derivative neurospheres, and single-cell gene expression profiles to define three tumor-intrinsic transcriptional subtypes designated as proneural, mesenchymal, and classical. Transcriptomic subtype multiplicity correlated with increased intratumoral heterogeneity and presence of tumor microenvironment. In silico cell sorting identified macrophages/microglia, CD4+ T lymphocytes, and neutrophils in the glioma microenvironment. NF1 deficiency resulted in increased tumor-associated macrophages/microglia infiltration. Longitudinal transcriptome analysis showed that expression subtype is retained in 55% of cases. Gene signature-based tumor microenvironment inference revealed a decrease in invading monocytes and a subtype-dependent increase in macrophages/microglia cells upon disease recurrence. Hypermutation at diagnosis or at recurrence associated with CD8+ T cell enrichment. Frequency of M2 macrophages detection associated with short-term relapse after radiation therapy.}, added-at = {2017-09-10T22:54:44.000+0200}, author = {Wang, Qianghu and Hu, Baoli and Hu, Xin and Kim, Hoon and Squatrito, Massimo and Scarpace, Lisa and deCarvalho, Ana C. and Lyu, Sali and Li, Pengping and Li, Yan and Barthel, Floris and Cho, Hee Jin and Lin, Yu-Hsi and Satani, Nikunj and Martinez-Ledesma, Emmanuel and Zheng, Siyuan and Chang, Edward and Sauvé, Charles-Etienne Gabriel and Olar, Adriana and Lan, Zheng D. and Finocchiaro, Gaetano and Phillips, Joanna J. and Berger, Mitchel S. and Gabrusiewicz, Konrad R. and Wang, Guocan and Eskilsson, Eskil and Hu, Jian and Mikkelsen, Tom and DePinho, Ronald A. and Muller, Florian and Heimberger, Amy B. and Sulman, Erik P. and Nam, Do-Hyun and Verhaak, Roel G.W}, biburl = {https://www.bibsonomy.org/bibtex/21f39100b25988e5e8ac9c576ff691a74/marcsaric}, booktitle = {Cancer Cell}, comment = {doi: 10.1016/j.ccell.2017.06.003}, description = {Tumor Evolution of Glioma-Intrinsic Gene Expression Subtypes Associates with Immunological Changes in the Microenvironment: Cancer Cell}, doi = {10.1016/j.ccell.2017.06.003}, interhash = {38735008fc5342054247e42fb6bc865f}, intrahash = {1f39100b25988e5e8ac9c576ff691a74}, issn = {15356108}, journal = {Cancer Cell}, keywords = {PAYWALL cancer glioma microenvironment}, number = 1, pages = {42--56.e6}, publisher = {Elsevier}, timestamp = {2017-09-10T22:55:17.000+0200}, title = {Tumor Evolution of Glioma-Intrinsic Gene Expression Subtypes Associates with Immunological Changes in the Microenvironment}, url = {http://dx.doi.org/10.1016/j.ccell.2017.06.003}, volume = 32, year = 2017 }