%0 Journal Article %1 Muller:2017:New-Phytol:28670699 %A Müller, H M %A Schäfer, N %A Bauer, H %A Geiger, D %A Lautner, S %A Fromm, J %A Riederer, M %A Bueno, A %A Nussbaumer, T %A Mayer, K %A Alquraishi, S A %A Alfarhan, A H %A Neher, E %A Al-Rasheid, K A S %A Ache, P %A Hedrich, R %D 2017 %J New Phytol %K Phoenix anion-channel dactylifera date-palm guard-cell slac1 %R 10.1111/nph.14672 %T The desert plant Phoenix dactylifera closes stomata via nitrate-regulated SLAC1 anion channel %U https://www.ncbi.nlm.nih.gov/pubmed/28670699 %X Date palm Phoenix dactylifera is a desert crop well adapted to survive and produce fruits under extreme drought and heat. How are palms under such harsh environmental conditions able to limit transpirational water loss? Here, we analysed the cuticular waxes, stomata structure and function, and molecular biology of guard cells from P.�dactylifera. To understand the stomatal response to the water stress phytohormone of the desert plant, we cloned the major elements necessary for guard cell fast abscisic acid (ABA) signalling and reconstituted this ABA signalosome in Xenopus oocytes. The PhoenixSLAC1-type anion channel is regulated by ABA kinase PdOST1. Energy-dispersive X-ray analysis (EDXA) demonstrated that date palm guard cells release chloride during stomatal closure. However, in Cl(-) medium, PdOST1 did not activate the desert plant anion channel PdSLAC1 per�se. Only when nitrate was present at the extracellular face of the anion channel did the OST1-gated PdSLAC1 open, thus enabling chloride release. In the presence of nitrate, ABA enhanced and accelerated stomatal closure. Our findings indicate that, in date palm, the guard cell osmotic motor driving stomatal closure uses nitrate as the signal to open the major anion channel SLAC1. This initiates guard cell depolarization and the release of anions together with potassium.

@article{Muller:2017:New-Phytol:28670699, abstract = {Date palm Phoenix dactylifera is a desert crop well adapted to survive and produce fruits under extreme drought and heat. How are palms under such harsh environmental conditions able to limit transpirational water loss? Here, we analysed the cuticular waxes, stomata structure and function, and molecular biology of guard cells from P.�dactylifera. To understand the stomatal response to the water stress phytohormone of the desert plant, we cloned the major elements necessary for guard cell fast abscisic acid (ABA) signalling and reconstituted this ABA signalosome in Xenopus oocytes. The PhoenixSLAC1-type anion channel is regulated by ABA kinase PdOST1. Energy-dispersive X-ray analysis (EDXA) demonstrated that date palm guard cells release chloride during stomatal closure. However, in Cl(-) medium, PdOST1 did not activate the desert plant anion channel PdSLAC1 per�se. Only when nitrate was present at the extracellular face of the anion channel did the OST1-gated PdSLAC1 open, thus enabling chloride release. In the presence of nitrate, ABA enhanced and accelerated stomatal closure. Our findings indicate that, in date palm, the guard cell osmotic motor driving stomatal closure uses nitrate as the signal to open the major anion channel SLAC1. This initiates guard cell depolarization and the release of anions together with potassium.}, added-at = {2017-08-04T12:07:34.000+0200}, author = {M{\"u}ller, H M and Sch{\"a}fer, N and Bauer, H and Geiger, D and Lautner, S and Fromm, J and Riederer, M and Bueno, A and Nussbaumer, T and Mayer, K and Alquraishi, S A and Alfarhan, A H and Neher, E and Al-Rasheid, K A S and Ache, P and Hedrich, R}, biburl = {https://www.bibsonomy.org/bibtex/26d035b7923d34acc2b934907d9e54b5e/rainerhedrich}, description = {The desert plant Phoenix dactylifera closes stomata via nitrate-regulated SLAC1 anion channel. - PubMed - NCBI}, doi = {10.1111/nph.14672}, interhash = {3a18fd03789343404665442cb2f715c7}, intrahash = {6d035b7923d34acc2b934907d9e54b5e}, journal = {New Phytol}, keywords = {Phoenix anion-channel dactylifera date-palm guard-cell slac1}, month = jul, pmid = {28670699}, timestamp = {2017-08-04T12:12:44.000+0200}, title = {The desert plant Phoenix dactylifera closes stomata via nitrate-regulated SLAC1 anion channel}, url = {https://www.ncbi.nlm.nih.gov/pubmed/28670699}, year = 2017 }