Zusammenfassung
Antagonist activities of different Trichoderma strains were evaluated in vitro against Colletotrichum capsici, a causal agent of anthracnose fruit rot of chili. Dual culture test showed that Trichoderma strains effectively inhibited mycelia growth of the pathogen. T. harzianum IMI-392433 showed the highest inhibition (81.96 %) and mycelial overgrowth (78.98%). Also, metabolites having 80% concentration extracted from 30-day-old T. harzianum IMI-392433 revealed the highest PIRG (percentage inhibition of radial growth) value of 85.16 and 87.18% by using normal poison and
modified bilayer poison agar technique, respectively. Further, metabolites extracted from 30-day-old T. harzianum IMI-392433 at a concentration of 2000 mgL-1 completely inhibited spore germination and germ tube elongation of the pathogen. Also, severity of anthracnose was significantly decreased as compared with the control (2% methanol) when chili fruits were soaked in 2000 mgL-1 of 30-day-old
metabolites from T. harzianum strains. Importantly, metabolites extracted from T. virens IMI-392430 (71.09) and T. pseudokoningii IMI-392431 (69.52) were found to be most efficient in the inhibition of disease severity. Taken together, our data suggest that Trichoderma strains especially T. harzianum IMI-392433 is a potential antagonistic organism and can be used to control the anthracnose disease
caused by C. capsici.
Nutzer