%0 Journal Article %1 dashti2014production %A Dashti, Y %A Grkovic, T %A Abdelmohsen, Usama Ramadan %A Hentschel, Ute %A Quinn, R J %D 2014 %J Mar. Drugs %K myown %N 5 %P 3046-3059 %T Production of induced secondary metabolites by a co-culture of sponge-associated actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 %U http://dx.doi.org/10.3390/md12053046 %V 12 %X Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by ¹H NMR. Ten known compounds, including angucycline, diketopiperazine and β-carboline derivatives 1-10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-2-hydroxyphenyl-acetamide 11, 1,6-dihydroxyphenazine 12 and 5a,6,11a,12-tetrahydro-5a,11a-dimethyl1,4benzoxazino3,2-b1,4benzoxazine 13a. When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes.

@article{dashti2014production, abstract = {Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by ¹H NMR. Ten known compounds, including angucycline, diketopiperazine and β-carboline derivatives 1-10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-2-hydroxyphenyl-acetamide 11, 1,6-dihydroxyphenazine 12 and 5a,6,11a,12-tetrahydro-5a,11a-dimethyl1,4benzoxazino3,2-b1,4benzoxazine 13a. When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes.}, added-at = {2014-04-23T16:13:25.000+0200}, author = {Dashti, Y and Grkovic, T and Abdelmohsen, Usama Ramadan and Hentschel, Ute and Quinn, R J}, biburl = {https://www.bibsonomy.org/bibtex/2d928045275cd4b067685b5bc66f1e0e7/abdelmohsen}, interhash = {564786204d02ddd66bf74345d0f23bb6}, intrahash = {d928045275cd4b067685b5bc66f1e0e7}, journal = {Mar. Drugs}, keywords = {myown}, number = 5, pages = {3046-3059}, timestamp = {2014-07-22T15:02:20.000+0200}, title = {Production of induced secondary metabolites by a co-culture of sponge-associated actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163}, url = {http://dx.doi.org/10.3390/md12053046}, volume = 12, year = 2014 }