Abstract
cAMP is an important second messenger with a plethora of cellular
effects and biological roles. To monitor and visualize cAMP in intact
living cells, electrophysiological and fluorescent methods have been
developed based on activation of all three types of cAMP effectors:
protein kinase A, cyclic nucleotide-gated channels, and exchange
protein directly activated by cAMP. In this review, we describe and
compare these techniques in terms of their robustness, sensitivity
and spatio-temporal resolution.
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