Abstract
A method is presented for recording extrinsic optical signals from
segments of single skeletal muscle fibers under current or voltage
clamp conditions. Such segments, which are cut from intact fibers,
are maintained in a relaxed state, while exhbiting otherwise normal
physiological properties, including healthy delayed rectifier currents.
Extrinsic fluorescence changes are demonstrated, using the permeant
potentiometric probe, Nile Blue A. These changes vary nonlinearly
with the controlled surface membrane potential, in a manner which
suggests that they arise from potential changes in the sarcoplasmic
reticulum. According to this interpretation, a simple model based
on the gating charge movement implicated in excitation-contraction
coupling, provides a self-consistent description of the voltage dependence
of the signal that requires no additional parameters.
- 310445
- action
- animals,
- anura,
- catesbeiana,
- contraction,
- dyes,
- fluorescence,
- fluorescent
- gov't,
- muscle
- muscles,
- non-p.h.s.,
- potentials,
- rana
- research
- support,
- u.s.
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