%0 Journal Article %1 huhnstock2022threedimensional %A Huhnstock, Rico %A Reginka, Meike %A Sonntag, Claudius %A Merkel, Maximilian %A Dingel, Kristina %A Sick, Bernhard %A Vogel, Michael %A Ehresmann, Arno %D 2022 %J Scientific Reports %K imported %N 1 %P 20890-- %R 10.1038/s41598-022-25391-z %T Three-dimensional close-to-substrate trajectories of magnetic microparticles in dynamically changing magnetic field landscapes %U https://doi.org/10.1038/s41598-022-25391-z %V 12 %X The transport of magnetic particles (MPs) by dynamic magnetic field landscapes (MFLs) using magnetically patterned substrates is promising for the development of Lab-on-a-chip (LOC) systems. The inherent close-to-substrate MP motion is sensitive to changing particle–substrate interactions. Thus, the detection of a modified particle–substrate separation distance caused by surface binding of an analyte is expected to be a promising probe in analytics and diagnostics. Here, we present an essential prerequisite for such an application, namely the label-free quantitative experimental determination of the three-dimensional trajectories of superparamagnetic particles (SPPs) transported by a dynamically changing MFL. The evaluation of defocused SPP images from optical bright-field microscopy revealed a “hopping”-like motion of the magnetic particles, previously predicted by theory, additionally allowing a quantification of maximum jump heights. As our findings pave the way towards precise determination of particle–substrate separations, they bear deep implications for future LOC detection schemes using only optical microscopy.

@article{huhnstock2022threedimensional, abstract = {The transport of magnetic particles (MPs) by dynamic magnetic field landscapes (MFLs) using magnetically patterned substrates is promising for the development of Lab-on-a-chip (LOC) systems. The inherent close-to-substrate MP motion is sensitive to changing particle–substrate interactions. Thus, the detection of a modified particle–substrate separation distance caused by surface binding of an analyte is expected to be a promising probe in analytics and diagnostics. Here, we present an essential prerequisite for such an application, namely the label-free quantitative experimental determination of the three-dimensional trajectories of superparamagnetic particles (SPPs) transported by a dynamically changing MFL. The evaluation of defocused SPP images from optical bright-field microscopy revealed a “hopping”-like motion of the magnetic particles, previously predicted by theory, additionally allowing a quantification of maximum jump heights. As our findings pave the way towards precise determination of particle–substrate separations, they bear deep implications for future LOC detection schemes using only optical microscopy.}, added-at = {2024-02-07T11:52:21.000+0100}, author = {Huhnstock, Rico and Reginka, Meike and Sonntag, Claudius and Merkel, Maximilian and Dingel, Kristina and Sick, Bernhard and Vogel, Michael and Ehresmann, Arno}, biburl = {https://www.bibsonomy.org/bibtex/29dbc39c808649c1a4b268751b56e0879/aimed}, doi = {10.1038/s41598-022-25391-z}, interhash = {77f498d34493e1e432bac8b420d385be}, intrahash = {9dbc39c808649c1a4b268751b56e0879}, issn = {20452322}, journal = {Scientific Reports}, keywords = {imported}, number = 1, pages = {20890--}, refid = {Huhnstock2022}, timestamp = {2024-02-07T11:52:21.000+0100}, title = {Three-dimensional close-to-substrate trajectories of magnetic microparticles in dynamically changing magnetic field landscapes}, url = {https://doi.org/10.1038/s41598-022-25391-z}, volume = 12, year = 2022 }