%0 Journal Article %1 Arifuzzaman2006Largescale %A Arifuzzaman, Mohammad %A Maeda, Maki %A Itoh, Aya %A Nishikata, Kensaku %A Takita, Chiharu %A Saito, Rintaro %A Ara, Takeshi %A Nakahigashi, Kenji %A Huang, Hsuan-Cheng C. %A Hirai, Aki %A Tsuzuki, Kohei %A Nakamura, Seira %A Altaf-Ul-Amin, Mohammad %A Oshima, Taku %A Baba, Tomoya %A Yamamoto, Natsuko %A Kawamura, Tomoyo %A Ioka-Nakamichi, Tomoko %A Kitagawa, Masanari %A Tomita, Masaru %A Kanaya, Shigehiko %A Wada, Chieko %A Mori, Hirotada %C Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Japan; %D 2006 %J Genome research %K protein-protein-interactions %N 5 %P 686--691 %R 10.1101/gr.4527806 %T Large-scale identification of protein-protein interaction of Escherichia coli K-12. %U http://dx.doi.org/10.1101/gr.4527806 %V 16 %X Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested, partners were found for 2667, including 779 of unknown function. Proteins copurifying with hexahistidine-tagged baits on a Ni2+-NTA column were identified by MALDI-TOF MS (matrix-assisted laser desorption ionization time of flight mass spectrometry). An extended analysis of these interacting networks by bioinformatics and experimentation should provide new insights and novel strategies for E. coli systems biology.

@article{Arifuzzaman2006Largescale, abstract = {Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli {ORF} clone library. Of 4339 bait proteins tested, partners were found for 2667, including 779 of unknown function. Proteins copurifying with hexahistidine-tagged baits on a {Ni2+-NTA} column were identified by {MALDI}-{TOF} {MS} (matrix-assisted laser desorption ionization time of flight mass spectrometry). An extended analysis of these interacting networks by bioinformatics and experimentation should provide new insights and novel strategies for E. coli systems biology.}, added-at = {2018-12-02T16:09:07.000+0100}, address = {Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Japan;}, author = {Arifuzzaman, Mohammad and Maeda, Maki and Itoh, Aya and Nishikata, Kensaku and Takita, Chiharu and Saito, Rintaro and Ara, Takeshi and Nakahigashi, Kenji and Huang, Hsuan-Cheng C. and Hirai, Aki and Tsuzuki, Kohei and Nakamura, Seira and Altaf-Ul-Amin, Mohammad and Oshima, Taku and Baba, Tomoya and Yamamoto, Natsuko and Kawamura, Tomoyo and Ioka-Nakamichi, Tomoko and Kitagawa, Masanari and Tomita, Masaru and Kanaya, Shigehiko and Wada, Chieko and Mori, Hirotada}, biburl = {https://www.bibsonomy.org/bibtex/23a0062c2b26ca763552fadc761f2a0e6/karthikraman}, citeulike-article-id = {591968}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gr.4527806}, citeulike-linkout-1 = {http://genome.cshlp.org/content/16/5/686.abstract}, citeulike-linkout-2 = {http://genome.cshlp.org/content/16/5/686.full.pdf}, citeulike-linkout-3 = {http://www.genome.org/cgi/content/abstract/16/5/686}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/16606699}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=16606699}, day = 10, doi = {10.1101/gr.4527806}, interhash = {78c77120eec5357e49929e547c993cf3}, intrahash = {3a0062c2b26ca763552fadc761f2a0e6}, issn = {1088-9051}, journal = {Genome research}, keywords = {protein-protein-interactions}, month = may, number = 5, pages = {686--691}, pmid = {16606699}, posted-at = {2009-11-14 19:03:23}, priority = {2}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Large-scale identification of protein-protein interaction of Escherichia coli K-12.}, url = {http://dx.doi.org/10.1101/gr.4527806}, volume = 16, year = 2006 }