%0 Journal Article %1 citeulike:471085 %A Massen, Mar %A Liu, Zheng %A Haruyama, Tetsuya %A Kobatake, Eiry %A Ikariyama, Yoshihito %A Aizawa, Masuo %D 1995 %J Analytica Chimica Acta %K amperometry fia immunoassay ag_detection heterogeneous %N 3 %P 353--359 %R 10.1016/0003-2670(94)00645-3 %T Immunosensing with amperometric detection, using galactosidase as label and as substrate %U http://www.sciencedirect.com/science/article/B6TF4-3YKKPWK-8W/2/6c0722c5716397552432184c88c21f84 %V 304 %X (PAPG) was shown to be a suitable substrate for the amperometric detection of galactosidase activity at neutral pH. The application of this amplification system for immunoassay was demonstrated. The product of the enzyme reaction, p-aminophenol (PAP), was detected at 200 mV, vs. Ag/AgCl, by flow-injection analysis (FIA), with a 50 nM detection limit. PAPG was hydrolyzed more than 2.5 times faster than , by the enzyme. Both PAP and PAPG were stable at pH 7. The galactosidase concentration could be measured down to a concentration of 100 fM, and mouse IgG could be assayed by sandwich immunoassay down to 700 fM. PAPG was found to be a promising reagent for heterogeneous systems, like the one described, and for homogenous assays of biological fluids.

@article{citeulike:471085, abstract = {(PAPG) was shown to be a suitable substrate for the amperometric detection of galactosidase activity at neutral pH. The application of this amplification system for immunoassay was demonstrated. The product of the enzyme reaction, p-aminophenol (PAP), was detected at 200 mV, vs. Ag/AgCl, by flow-injection analysis (FIA), with a 50 nM detection limit. PAPG was hydrolyzed more than 2.5 times faster than , by the enzyme. Both PAP and PAPG were stable at pH 7. The galactosidase concentration could be measured down to a concentration of 100 fM, and mouse IgG could be assayed by sandwich immunoassay down to 700 fM. PAPG was found to be a promising reagent for heterogeneous systems, like the one described, and for homogenous assays of biological fluids.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Massen, Mar and Liu, Zheng and Haruyama, Tetsuya and Kobatake, Eiry and Ikariyama, Yoshihito and Aizawa, Masuo}, biburl = {https://www.bibsonomy.org/bibtex/273c5e495d6d00a1775312d6af9d71a79/biblio24}, citeulike-article-id = {471085}, doi = {10.1016/0003-2670(94)00645-3}, interhash = {81366ca2fe268f3bc98270f72db711ff}, intrahash = {73c5e495d6d00a1775312d6af9d71a79}, journal = {Analytica Chimica Acta}, keywords = {amperometry fia immunoassay ag_detection heterogeneous}, month = {April}, number = 3, pages = {353--359}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Immunosensing with amperometric detection, using galactosidase as label and as substrate}, url = {http://www.sciencedirect.com/science/article/B6TF4-3YKKPWK-8W/2/6c0722c5716397552432184c88c21f84}, volume = 304, year = 1995 }