%0 Journal Article %1 Djuzenova2015346 %A Djuzenova, Cholpon S. %A Fiedler, Vanessa %A Memmel, Simon %A Katzer, Astrid %A Hartmann, Susanne %A Krohne, Georg %A Zimmermann, Heiko %A Scholz, Claus-Jürgen %A Polat, Bülent %A Flentje, Michael %A Sukhorukov, Vladimir L. %D 2015 %J Experimental Cell Research %K vladimir %N 2 %P 346 - 357 %R http://dx.doi.org/10.1016/j.yexcr.2014.08.013 %T Actin cytoskeleton organization, cell surface modification and invasion rate of 5 glioblastoma cell lines differing in \PTEN\ and p53 status %U http://www.sciencedirect.com/science/article/pii/S0014482714003413 %V 330 %X Glioblastoma cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores the relationship between the invasion capacity of 5 glioblastoma cell lines differing in p53 and PTEN status, expression of mTOR and several other marker proteins involved in cell invasion, actin cytoskeleton organization and cell morphology. We found that two glioblastoma lines mutated in both p53 and PTEN genes (U373-MG and SNB19) exhibited the highest invasion rates through the Matrigel or collagen matrix. In DK-MG (p53wt/PTENwt) and GaMG (p53mut/PTENwt) cells, F-actin mainly occurred in the numerous stress fibers spanning the cytoplasm, whereas U87-MG (p53wt/PTENmut), U373-MG and SNB19 (both p53mut/PTENmut) cells preferentially expressed F-actin in filopodia and lamellipodia. Scanning electron microscopy confirmed the abundant filopodia and lamellipodia in the PTEN mutated cell lines. Interestingly, the gene profiling analysis revealed two clusters of cell lines, corresponding to the most (U373-MG and SNB19, i.e. p53 and PTEN mutated cells) and less invasive phenotypes. The results of this study might shed new light on the mechanisms of glioblastoma invasion.

@article{Djuzenova2015346, abstract = {Glioblastoma cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores the relationship between the invasion capacity of 5 glioblastoma cell lines differing in p53 and PTEN status, expression of mTOR and several other marker proteins involved in cell invasion, actin cytoskeleton organization and cell morphology. We found that two glioblastoma lines mutated in both p53 and PTEN genes (U373-MG and SNB19) exhibited the highest invasion rates through the Matrigel or collagen matrix. In DK-MG (p53wt/PTENwt) and GaMG (p53mut/PTENwt) cells, F-actin mainly occurred in the numerous stress fibers spanning the cytoplasm, whereas U87-MG (p53wt/PTENmut), U373-MG and SNB19 (both p53mut/PTENmut) cells preferentially expressed F-actin in filopodia and lamellipodia. Scanning electron microscopy confirmed the abundant filopodia and lamellipodia in the PTEN mutated cell lines. Interestingly, the gene profiling analysis revealed two clusters of cell lines, corresponding to the most (U373-MG and SNB19, i.e. p53 and PTEN mutated cells) and less invasive phenotypes. The results of this study might shed new light on the mechanisms of glioblastoma invasion.}, added-at = {2015-03-11T12:36:14.000+0100}, author = {Djuzenova, Cholpon S. and Fiedler, Vanessa and Memmel, Simon and Katzer, Astrid and Hartmann, Susanne and Krohne, Georg and Zimmermann, Heiko and Scholz, Claus-Jürgen and Polat, Bülent and Flentje, Michael and Sukhorukov, Vladimir L.}, biburl = {https://www.bibsonomy.org/bibtex/284b45fea1b55a3d4ef512fce093820b1/reichert}, doi = {http://dx.doi.org/10.1016/j.yexcr.2014.08.013}, interhash = {193a5999c3566024312e135b9d357edf}, intrahash = {84b45fea1b55a3d4ef512fce093820b1}, issn = {0014-4827}, journal = {Experimental Cell Research }, keywords = {vladimir}, number = 2, pages = {346 - 357}, timestamp = {2015-03-11T12:36:14.000+0100}, title = {Actin cytoskeleton organization, cell surface modification and invasion rate of 5 glioblastoma cell lines differing in \{PTEN\} and p53 status }, url = {http://www.sciencedirect.com/science/article/pii/S0014482714003413}, volume = 330, year = 2015 }