A beacon aptamer-based biosensor for the detection of thrombin was developed using electrochemical transduction method. Gold surface was modified with a beacon aptamer covalently linked at 5'-terminus with a linker containing a primary aliphatic amine. Methylene blue (MB) was intercalated into the beacon sequence, and used as an electrochemical marker. When the beacon aptamer immobilized on gold surface encounters thrombin, the hairpin forming beacon aptamer is conformationally changed to release the intercalated MB, resulting a decrease in electrical current intensity in voltamogram. The peak signal of the MB is clearly decreased by the binding of thrombin onto the beacon aptamer. The linear range of the signal was observed between 0 and 50.8 nM of thrombin with 0.999 correlation factor. This method was able to linearly and selectively detect thrombin with a detection limit of 11 nM.
%0 Journal Article
%1 citeulike:780925
%A Bang, G. S.
%A Cho, S.
%A Kim, B. G.
%C School of Chemical and Biological Engineering and Institute of Molecular Biology and Genetics, Seoul National University, Kwanak-ku, South Korea.
%D 2005
%J Biosens Bioelectron
%K aptamer surface electrochemistry
%N 6
%P 863--870
%R 10.1016/j.bios.2005.02.002
%T A novel electrochemical detection method for aptamer biosensors.
%U http://dx.doi.org/10.1016/j.bios.2005.02.002
%V 21
%X A beacon aptamer-based biosensor for the detection of thrombin was developed using electrochemical transduction method. Gold surface was modified with a beacon aptamer covalently linked at 5'-terminus with a linker containing a primary aliphatic amine. Methylene blue (MB) was intercalated into the beacon sequence, and used as an electrochemical marker. When the beacon aptamer immobilized on gold surface encounters thrombin, the hairpin forming beacon aptamer is conformationally changed to release the intercalated MB, resulting a decrease in electrical current intensity in voltamogram. The peak signal of the MB is clearly decreased by the binding of thrombin onto the beacon aptamer. The linear range of the signal was observed between 0 and 50.8 nM of thrombin with 0.999 correlation factor. This method was able to linearly and selectively detect thrombin with a detection limit of 11 nM.
@article{citeulike:780925,
abstract = {A beacon aptamer-based biosensor for the detection of thrombin was developed using electrochemical transduction method. Gold surface was modified with a beacon aptamer covalently linked at 5'-terminus with a linker containing a primary aliphatic amine. Methylene blue (MB) was intercalated into the beacon sequence, and used as an electrochemical marker. When the beacon aptamer immobilized on gold surface encounters thrombin, the hairpin forming beacon aptamer is conformationally changed to release the intercalated MB, resulting a decrease in electrical current intensity in voltamogram. The peak signal of the MB is clearly decreased by the binding of thrombin onto the beacon aptamer. The linear range of the signal was observed between 0 and 50.8 nM of thrombin with 0.999 correlation factor. This method was able to linearly and selectively detect thrombin with a detection limit of 11 nM.},
added-at = {2006-07-31T20:01:51.000+0200},
address = {School of Chemical and Biological Engineering and Institute of Molecular Biology and Genetics, Seoul National University, Kwanak-ku, South Korea.},
author = {Bang, G. S. and Cho, S. and Kim, B. G.},
biburl = {https://www.bibsonomy.org/bibtex/2dcb6d2e525838c045e874bea1847c145/biblio24},
citeulike-article-id = {780925},
doi = {10.1016/j.bios.2005.02.002},
interhash = {94be042629bb67c80e73e6897100040f},
intrahash = {dcb6d2e525838c045e874bea1847c145},
issn = {0956-5663},
journal = {Biosens Bioelectron},
keywords = {aptamer surface electrochemistry},
month = {December},
number = 6,
pages = {863--870},
priority = {2},
timestamp = {2006-07-31T20:01:51.000+0200},
title = {A novel electrochemical detection method for aptamer biosensors.},
url = {http://dx.doi.org/10.1016/j.bios.2005.02.002},
volume = 21,
year = 2005
}