%0 Journal Article %1 Eszlinger.2014 %A Eszlinger, Markus %A Krogdahl, Annelise %A Münz, Sina %A Rehfeld, Christian %A Precht Jensen, Eva Magrethe, %A Ferraz, Carolina %A Bösenberg, Eileen %A Drieschner, Norbert %A Scholz, Markus %A Hegedüs, Laszlo %A Paschke, Ralf %D 2014 %J Thyroid : official journal of the American Thyroid Association %K IMISE-Publikationen %N 2 %P 305–313 %T Impact of molecular screening for point mutations and rearrangements in routine air-dried fine-needle aspiration samples of thyroid nodules %V 24 %X BACKGROUND\r\nThe diagnostic limitations of thyroid fine-needle aspiration (FNA), such as the indeterminate category, can be partially overcome by molecular analyses. However, until now, rearrangements have only been detected in fresh FNA material and the number of follicular thyroid carcinomas (FTCs) was rather low in previous studies. We aimed at investigating the impact of point mutations and rearrangement detection in a set of routine air-dried FNA smears with a higher percentage of FTCs.\r\nMETHODS\r\nRNA and DNA was extracted from 310 FNAs (164 indeterminate, 57 malignant, 89 benign) and corresponding formalin-fixed paraffin-embedded tissue (156 follicular adenomas FAs, 32 FTCs, 44 papillary thyroid carcinomas PTCs, 9 follicular variant PTCs, and 69 goiters). PAX8/PPARG and RET/PTC rearrangements were detected by qPCR, BRAF and RAS mutations by high-resolution melting PCR and by pyrosequencing.\r\nRESULTS\r\nForty-seven mutations were detected in the FNAs: 22 BRAF, 13 NRAS, and 3 HRAS mutations, 8 PAX8/PPARG, and one RET/PTC-rearrangement. While the presence of a BRAF and RET/PTC mutation was associated with cancer in 100\% of samples each, the presence of a RAS and PAX8/PPARG mutation was associated with cancer in only 12\% and 50\% of samples, respectively. In the indeterminate group 4 of 25 carcinomas were identified by molecular FNA screening, which increased the sensitivity from 67\% (cytology alone) to 75\% (cytology plus molecular screening).\r\nCONCLUSION\r\nMolecular screening for point mutations and rearrangements is feasible in air-dried FNAs. Although the impact of detecting point mutations and rearrangements in FNAs has most likely been overestimated in previous studies, molecular FNA analyses improve presurgical diagnostics. The detection of BRAF mutations in FNA may improve the choice of surgery and postsurgical treatment. Further data are necessary to elucidate the true impact of detecting RAS and PAX8/PPARG mutations in FNAs. The inclusion of additional rare somatic mutations and miRNA markers might further improve the impact of molecular FNA diagnostics.

@article{Eszlinger.2014, abstract = {BACKGROUND\r\nThe diagnostic limitations of thyroid fine-needle aspiration (FNA), such as the indeterminate category, can be partially overcome by molecular analyses. However, until now, rearrangements have only been detected in fresh FNA material and the number of follicular thyroid carcinomas (FTCs) was rather low in previous studies. We aimed at investigating the impact of point mutations and rearrangement detection in a set of routine air-dried FNA smears with a higher percentage of FTCs.\r\nMETHODS\r\nRNA and DNA was extracted from 310 FNAs (164 indeterminate, 57 malignant, 89 benign) and corresponding formalin-fixed paraffin-embedded tissue (156 follicular adenomas [FAs], 32 FTCs, 44 papillary thyroid carcinomas [PTCs], 9 follicular variant PTCs, and 69 goiters). PAX8/PPARG and RET/PTC rearrangements were detected by qPCR, BRAF and RAS mutations by high-resolution melting PCR and by pyrosequencing.\r\nRESULTS\r\nForty-seven mutations were detected in the FNAs: 22 BRAF, 13 NRAS, and 3 HRAS mutations, 8 PAX8/PPARG, and one RET/PTC-rearrangement. While the presence of a BRAF and RET/PTC mutation was associated with cancer in 100\% of samples each, the presence of a RAS and PAX8/PPARG mutation was associated with cancer in only 12\% and 50\% of samples, respectively. In the indeterminate group 4 of 25 carcinomas were identified by molecular FNA screening, which increased the sensitivity from 67\% (cytology alone) to 75\% (cytology plus molecular screening).\r\nCONCLUSION\r\nMolecular screening for point mutations and rearrangements is feasible in air-dried FNAs. Although the impact of detecting point mutations and rearrangements in FNAs has most likely been overestimated in previous studies, molecular FNA analyses improve presurgical diagnostics. The detection of BRAF mutations in FNA may improve the choice of surgery and postsurgical treatment. Further data are necessary to elucidate the true impact of detecting RAS and PAX8/PPARG mutations in FNAs. The inclusion of additional rare somatic mutations and miRNA markers might further improve the impact of molecular FNA diagnostics.}, added-at = {2014-10-13T18:24:45.000+0200}, author = {Eszlinger, Markus and Krogdahl, Annelise and Münz, Sina and Rehfeld, Christian and {Precht Jensen, Eva Magrethe} and Ferraz, Carolina and Bösenberg, Eileen and Drieschner, Norbert and Scholz, Markus and Hegedüs, Laszlo and Paschke, Ralf}, biburl = {https://www.bibsonomy.org/bibtex/27a2a753496bf178c16185c5a344a4c57/drtester}, interhash = {9b5e201d867db9192c6774250e7e35d7}, intrahash = {7a2a753496bf178c16185c5a344a4c57}, journal = {Thyroid : official journal of the American Thyroid Association}, keywords = {IMISE-Publikationen}, number = 2, pages = {305–313}, timestamp = {2014-10-13T18:24:45.000+0200}, title = {Impact of molecular screening for point mutations and rearrangements in routine air-dried fine-needle aspiration samples of thyroid nodules}, volume = 24, year = 2014 }