%0 Journal Article %1 Lee1999 %A Lee, S. Y. %A Lee, Y. %A Wang, F. %D 1999 %J Biotechnol. Bioeng., %K 10486136 Bacteria, Carbohydrates, Chromatography, High Liquid, Plastics, Polyesters, Pressure Stereoisomerism, %N 3 %P 363-8 %T Chiral compounds from bacterial polyesters: sugars to plastics to fine chemicals. %V 65 %X A novel and efficient method for the production of enantiomerically pure (R)-(-)-hydroxycarboxylic acids by in vivo depolymerization of microbial polyester polyhydroxyalkanoates (PHAs) was developed. Using this method, several model compounds, (R)-(-)-3-hydroxyalkanoic acids, consisting of 4 to 12 carbon atoms, and (R)-(-)-3-hydroxy-5-phenylvaleric acid, could be prepared. In particular, (R)-(-)-3-hydroxybutyric acid could be efficiently prepared by this method. By providing the environmental condition in which cells possess high activity of intracellular PHA depolymerase and low activity of (R)-(-)-3-hydroxybutyric acid dehydrogenase, (R)-(-)-3-hydroxybutyric acid could be produced with a yield of 96\% in only 30 min by in vivo depolymerization of polyhydroxybutyrate (PHB) accumulated in Alcaligenes latus.

@article{Lee1999, abstract = {A novel and efficient method for the production of enantiomerically pure (R)-(-)-hydroxycarboxylic acids by in vivo depolymerization of microbial polyester polyhydroxyalkanoates (PHAs) was developed. Using this method, several model compounds, (R)-(-)-3-hydroxyalkanoic acids, consisting of 4 to 12 carbon atoms, and (R)-(-)-3-hydroxy-5-phenylvaleric acid, could be prepared. In particular, (R)-(-)-3-hydroxybutyric acid could be efficiently prepared by this method. By providing the environmental condition in which cells possess high activity of intracellular PHA depolymerase and low activity of (R)-(-)-3-hydroxybutyric acid dehydrogenase, (R)-(-)-3-hydroxybutyric acid could be produced with a yield of 96\% in only 30 min by in vivo depolymerization of polyhydroxybutyrate (PHB) accumulated in Alcaligenes latus.}, added-at = {2010-12-02T09:30:05.000+0100}, author = {Lee, S. Y. and Lee, Y. and Wang, F.}, biburl = {https://www.bibsonomy.org/bibtex/256dc39fa7bacebece7decb09101346a7/afranz}, interhash = {ad2c6b8a884361fd2dc75530e4eb4b5e}, intrahash = {56dc39fa7bacebece7decb09101346a7}, journal = {Biotechnol. Bioeng.,}, keywords = {10486136 Bacteria, Carbohydrates, Chromatography, High Liquid, Plastics, Polyesters, Pressure Stereoisomerism,}, month = Nov, number = 3, pages = {363-8}, pii = {3.0.CO;2-1}, timestamp = {2010-12-02T09:30:07.000+0100}, title = {Chiral compounds from bacterial polyesters: sugars to plastics to fine chemicals.}, volume = 65, year = 1999 }