Аннотация
Pasteurella multocida toxin (PMT) is a highly potent mitogen for a
variety of cell types. PMT has been shown to induce various cellular
signaling processes, and it has been suggested to function through
the heterotrimeric G-proteins G(q)/G(11). To analyze the role of
G(q)/G(11) in the action of PMT, we have studied the effect of the
toxin in Galpha(q)/Galpha(11) double-deficient fibroblasts as well
as in fibroblasts lacking only Galpha(q) or Galpha(11). Interestingly,
formation of inositol phosphates in response to PMT was exclusively
dependent on Galpha(q) but not on the closely related Galpha(11).
Although Galpha(q)/Galpha(11) double-deficient and Galpha(q)-deficient
cells did not respond with any production of inositol phosphates
to PMT, PMT was still able to induce various other cellular effects
in these cells, including the activation of Rho, the Rho-dependent
formation of actin stress fibers and focal adhesions, as well as
the stimulation of c-Jun N-terminal kinase and extracellular signal-regulated
kinase. These data show that PMT leads to a variety of cellular effects
that are mediated only in part by the heterotrimeric G-protein G(q).
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