Article,

Effective Biosurfactants production by Pseudomonas aeruginosa and its efficacy on different Oils

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Journal of Advanced Laboratory Research in Biology, 1 (1): 31-34 (2010)

Abstract

A rhamnolipid producing bacterium, Pseudomonas aeruginosa was isolated from contaminated soil with oily wastes. The Pseudomonas aeruginosa has grown with glucose and corn oil as a carbon source produced biosurfactant. This biosurfactant was purified by procedures that included chloroform-ethanol extraction and 0.05M bicarbonate treatments. The active compound was identified as rhamnolipid by using thin layer chromatography. The biosurfactant efficacy was tested on coconut oil, groundnut oil, sunflower oil and olive oil. The coconut oil responded better and gave a maximum level of 1cm than the olive oil, groundnut oil and sunflower oil.

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