%0 Journal Article %1 citeulike:558313 %A Reade, V. %A Tailleux, A. %A Reade, R. %A Harduin, P. %A Cachera, C. %A Tacquet, A. %A Fruchart, J. C. %A Fievet, C. %C Laboratoire de Recherche Néphrologique, CHU de Lille, Hôpital A. Calmette, France. %D 1993 %J Kidney Int %K mapping epitope antibody apob %N 6 %P 1360--1365 %T Expression of apolipoprotein B epitopes in low density lipoproteins of hemodialyzed patients. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7508006 %V 44 %X Serum and isolated low-density lipoprotein (LDL) composition abnormalities were investigated in 20 hemodialyzed patients with chronic renal failure and 15 healthy normolipidemic subjects for comparison. LDL apolipoprotein B (apo B) epitope accessibility was determined by the use of seven monoclonal antibodies (Mabs). These Mabs recognize fragments on the N-terminal part of apo B (Mabs B1, B4), on the middle part (Mab BL7), on the C-terminal (Mabs BA11, BL3), and the two remaining Mabs recognize conformational epitopes of apo B (BL5, DA7). Mab BA11 recognizes a fragment of apo B which interacts with the B/E receptor. In hemodialyzed patients, LDL content of triglycerides (P < 0.001) and apo CIII (P < 0.005) was increased, while cholesteryl esters (P < 0.005) were decreased. The accessibility of BL5 epitopes of LDL apo B was enhanced (P < 0.05), while BA11 epitope expression was decreased (P < 0.01). The conformation of patients' LDL (CRF-LDL) was probably abnormal and seemed to be related to some modification of the lipidic environment. It is important to consider a structural modification as it alters the B/E receptor recognition domain of apo B. These results confirm LDL abnormalities in hemodialyzed patients and suggest a possible modification of the recognition of the LDL by cells.

@article{citeulike:558313, abstract = {Serum and isolated low-density lipoprotein (LDL) composition abnormalities were investigated in 20 hemodialyzed patients with chronic renal failure and 15 healthy normolipidemic subjects for comparison. LDL apolipoprotein B (apo B) epitope accessibility was determined by the use of seven monoclonal antibodies (Mabs). These Mabs recognize fragments on the N-terminal part of apo B (Mabs B1, B4), on the middle part (Mab BL7), on the C-terminal (Mabs BA11, BL3), and the two remaining Mabs recognize conformational epitopes of apo B (BL5, DA7). Mab BA11 recognizes a fragment of apo B which interacts with the B/E receptor. In hemodialyzed patients, LDL content of triglycerides (P < 0.001) and apo CIII (P < 0.005) was increased, while cholesteryl esters (P < 0.005) were decreased. The accessibility of BL5 epitopes of LDL apo B was enhanced (P < 0.05), while BA11 epitope expression was decreased (P < 0.01). The conformation of patients' LDL (CRF-LDL) was probably abnormal and seemed to be related to some modification of the lipidic environment. It is important to consider a structural modification as it alters the B/E receptor recognition domain of apo B. These results confirm LDL abnormalities in hemodialyzed patients and suggest a possible modification of the recognition of the LDL by cells.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Laboratoire de Recherche Néphrologique, CHU de Lille, Hôpital A. Calmette, France.}, author = {Reade, V. and Tailleux, A. and Reade, R. and Harduin, P. and Cachera, C. and Tacquet, A. and Fruchart, J. C. and Fievet, C.}, biburl = {https://www.bibsonomy.org/bibtex/2fecdb0fd297c7fe9a5bb88d0ae4c4932/biblio24}, citeulike-article-id = {558313}, interhash = {ec1cdcc6b84f099e6fa1760939698694}, intrahash = {fecdb0fd297c7fe9a5bb88d0ae4c4932}, issn = {0085-2538}, journal = {Kidney Int}, keywords = {mapping epitope antibody apob}, month = {December}, number = 6, pages = {1360--1365}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Expression of apolipoprotein B epitopes in low density lipoproteins of hemodialyzed patients.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=7508006}, volume = 44, year = 1993 }