Abstract
Three non-ribosomal peptide synthetase genes, termed sidD, sidC and
sidE, have been identified in Aspergillus fumigatus. Gene expression
analysis by RT-PCR confirms that expression of both sidD and C was
reduced by up to 90% under iron-replete conditions indicative of
a likely role in siderophore biosynthesis. SidE expression was less
sensitive to iron levels. In addition, two proteins purified from
mycelia grown under iron-limiting conditions corresponded to SidD
( approximately 200 kDa) and SidC (496 kDa) as determined by MALDI
ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore
synthetases are unique in bacteria and fungi and represent an attractive
target for antimicrobial chemotherapy.
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