Chip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue for normal operation, this study presents a system model of the real-time Polymerase Chain Reaction (PCR) machine to analyze the instrument design through numerical experiments. Based on model analysis, a systematic approach was developed to lower the variation of DNA quantification and achieve a robust design for a real-time PCR-on-a-chip system. Accelerated lift testing was adopted to evaluate the reliability of the chip prototype. According to the life test plan, this proposed real-time PCR-on-a-chip system was simulated to work continuously for over three years with similar reproducibility in DNA quantification. This not only shows the robustness of the lab-on-a-chip system, but also verifies the effectiveness of our systematic method for achieving a robust design.
%0 Journal Article
%1 Lee2010
%A Lee, Da-Sheng
%C KANDERERSTRASSE 25, CH-4057 BASEL, SWITZERLAND
%D 2010
%I MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL-MDPI
%J SENSORS
%K iontek
%N 1
%P 697-718
%R 10.3390/s100100697
%T Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System
%V 10
%X Chip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue for normal operation, this study presents a system model of the real-time Polymerase Chain Reaction (PCR) machine to analyze the instrument design through numerical experiments. Based on model analysis, a systematic approach was developed to lower the variation of DNA quantification and achieve a robust design for a real-time PCR-on-a-chip system. Accelerated lift testing was adopted to evaluate the reliability of the chip prototype. According to the life test plan, this proposed real-time PCR-on-a-chip system was simulated to work continuously for over three years with similar reproducibility in DNA quantification. This not only shows the robustness of the lab-on-a-chip system, but also verifies the effectiveness of our systematic method for achieving a robust design.
@article{Lee2010,
abstract = {{Chip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue for normal operation, this study presents a system model of the real-time Polymerase Chain Reaction (PCR) machine to analyze the instrument design through numerical experiments. Based on model analysis, a systematic approach was developed to lower the variation of DNA quantification and achieve a robust design for a real-time PCR-on-a-chip system. Accelerated lift testing was adopted to evaluate the reliability of the chip prototype. According to the life test plan, this proposed real-time PCR-on-a-chip system was simulated to work continuously for over three years with similar reproducibility in DNA quantification. This not only shows the robustness of the lab-on-a-chip system, but also verifies the effectiveness of our systematic method for achieving a robust design.}},
added-at = {2011-01-19T09:25:16.000+0100},
address = {{KANDERERSTRASSE 25, CH-4057 BASEL, SWITZERLAND}},
affiliation = {Lee, DS (Reprint Author), Natl Taipei Univ Technol, Dept Energy \& Refrigerating Air Conditioning Engn, Taipei, Taiwan. Natl Taipei Univ Technol, Dept Energy \& Refrigerating Air Conditioning Engn, Taipei, Taiwan.},
author = {Lee, Da-Sheng},
biburl = {https://www.bibsonomy.org/bibtex/200ef012b454ce0f49d079420f181b41b/hkayabilisim},
category = {Chemistry, Analytical; Electrochemistry; Instruments \& Instrumentation},
cited = {0},
doi = {10.3390/s100100697},
email = {f11167@ntut.edu.tw},
file = {:home/hkaya/Projeler/Robot/doc/literature/Lee2010.pdf:PDF},
id = {ISI:000273999800041},
interhash = {00fd3e9eec85a20f1feecfe7142d569b},
intrahash = {00ef012b454ce0f49d079420f181b41b},
iso = {Sensors},
issn = {1424-8220},
journal = {{SENSORS}},
keywords = {iontek},
language = {English},
month = {{JAN}},
number = {{1}},
pages = {{697-718}},
plus = {HEPATITIS-B-VIRUS; QUANTITATIVE PCR; HIGH-THROUGHPUT; AMPLIFICATION EFFICIENCY; DNA AMPLIFICATION; RAPID DETECTION; WASTE-WATER; QUANTIFICATION; FLUORESCENCE; BACTERIA},
publisher = {{MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL-MDPI}},
references = {46},
timestamp = {2011-01-19T09:25:17.000+0100},
title = {{Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System}},
type = {{Article}},
volume = {{10}},
year = {{2010}}
}